Structural characterization and antioxidative activity of low-molecular-weights beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies

Pai Feng Kao, Shwu Huey Wang, Wei Ting Hung, Yu Han Liao, Chun Mao Lin, Wen Bin Yang

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Abstract

The major cell wall constituent of Ganoderma lucidum (G. lucidum) is β-1,3-glucan. This study examined the polysaccharide from the residues of alkaline-extracted fruiting bodies using high-performance anion-exchange chromatography (HPAEC), and it employed nuclear magnetic resonance (NMR) and mass spectrometry (MS) to confirm the structures. We have successfully isolated low-molecular-weight β-1,3-glucan (LMG), in high yields, from the waste residue of extracted fruiting bodies of G. lucidum. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay evaluated the capability of LMG to suppress H 2O 2-induced cell death in RAW264.7 cells, identifying that LMG protected cells from H 2O 2-induced damage. LMG treatment decreased H 2O 2-induced intracellular reactive oxygen species (ROS) production. LMG also influenced sphingomyelinase (SMase) activity, stimulated by cell death to induce ceramide formation, and then increase cell ROS production. Estimation of the activities of neutral and acid SMases in vitro showed that LMG suppressed the activities of both neutral and acid SMases in a concentration-dependent manner. These results suggest that LMG, a water-soluble β-1,3-glucan recycled from extracted residue of G. lucidum, possesses antioxidant capability against H2O2-induced cell death by attenuating intracellular ROS and inhibiting SMase activity.

Original languageEnglish
Article number673764
JournalJournal of Biomedicine and Biotechnology
Volume2012
DOIs
Publication statusPublished - 2012

Fingerprint

Reishi
Glucans
Molecular Weight
Molecular weight
Cell death
Sphingomyelin Phosphodiesterase
Reactive Oxygen Species
Cell Death
beta-1,3-glucan
Acids
Ceramides
Chromatography
Cell Wall
Anions
Mass spectrometry
Polysaccharides
Assays
Mass Spectrometry
Magnetic Resonance Spectroscopy
Antioxidants

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

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title = "Structural characterization and antioxidative activity of low-molecular-weights beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies",
abstract = "The major cell wall constituent of Ganoderma lucidum (G. lucidum) is β-1,3-glucan. This study examined the polysaccharide from the residues of alkaline-extracted fruiting bodies using high-performance anion-exchange chromatography (HPAEC), and it employed nuclear magnetic resonance (NMR) and mass spectrometry (MS) to confirm the structures. We have successfully isolated low-molecular-weight β-1,3-glucan (LMG), in high yields, from the waste residue of extracted fruiting bodies of G. lucidum. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay evaluated the capability of LMG to suppress H 2O 2-induced cell death in RAW264.7 cells, identifying that LMG protected cells from H 2O 2-induced damage. LMG treatment decreased H 2O 2-induced intracellular reactive oxygen species (ROS) production. LMG also influenced sphingomyelinase (SMase) activity, stimulated by cell death to induce ceramide formation, and then increase cell ROS production. Estimation of the activities of neutral and acid SMases in vitro showed that LMG suppressed the activities of both neutral and acid SMases in a concentration-dependent manner. These results suggest that LMG, a water-soluble β-1,3-glucan recycled from extracted residue of G. lucidum, possesses antioxidant capability against H2O2-induced cell death by attenuating intracellular ROS and inhibiting SMase activity.",
author = "Kao, {Pai Feng} and Wang, {Shwu Huey} and Hung, {Wei Ting} and Liao, {Yu Han} and Lin, {Chun Mao} and Yang, {Wen Bin}",
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T1 - Structural characterization and antioxidative activity of low-molecular-weights beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies

AU - Kao, Pai Feng

AU - Wang, Shwu Huey

AU - Hung, Wei Ting

AU - Liao, Yu Han

AU - Lin, Chun Mao

AU - Yang, Wen Bin

PY - 2012

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