Strong association of antiepithelial cell antibodies with HLA-DR3 or DR7 phenotype in patients with recurrent oral ulcers

A. Sun, R. P. Hsieh, B. Y. Liu, J. T. Wang, J. S. Leu, Y. C. Wu, C. P. Chiang

Research output: Contribution to journalArticle

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Abstract

Background: Previous studies showed that antiepithelial cell antibodies (anti-ECA) were present in 71% (15/21) of patients with recurrent oral ulcers (ROU) and that there was a strong association of human leukocyte antigen (HLA)-DRw9 with ROU in Chinese patients. In this study, we assessed anti-ECA in a larger group of Chinese patients with ROU (n = 88) in order to further investigate the association of anti-ECA with HLA-DR and -DQ antigens. Methods: The anti-ECA in the sera of ROU patients were detected by an indirect immunofluorescence technique with rat esophagus as the substrate, and the HLA-DR and -DQ antigens in ROU patients were typed by a standard microcytotoxicity assay using Terasaki's oriental tray. Results: The rate of anti-ECA positivity was significantly higher (p < 0.0001) in ROU patients (68%) than in healthy control subjects (0%). Furthermore, the rate of anti-ECA positivity in patients with major or minor oral ulcers (72%) was significantly higher (29%) than that in patients with herpetiform ulcers (p < 0.05). There was a significant increase in the frequency of DR3 or DR7 antigen expression (p < 0.0001, p(c) [p corrected] < 0.001, relative risk [RR] = 4.3, etiologic fraction = 0.41) in anti-ECA-positive ROU patients compared with the corresponding frequencies in healthy control subjects. There was also a significant increase in the frequency of DR7 or DRw9 antigen expression (p < 0.005, p(c) < 0.05, RR = 4.7, etiologic fraction = 0.45) compared to healthy controls. Conclusions: Because only DR3 or DR7 antigen occurred more frequently in anti-ECA-positive than in anti-ECA-negative ROU patients (p < 0.0007, p(c) < 0.05, RR = 19.6, etiologic fraction = 0.51), we concluded that the gene coding for DR3 or DR7 antigen may contribute to the presence of anti-ECA in Chinese patients with ROU.

Original languageEnglish
Pages (from-to)290-294
Number of pages5
JournalJournal of the Formosan Medical Association
Volume99
Issue number4
Publication statusPublished - Sep 4 2000
Externally publishedYes

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Oral Ulcer
HLA Antigens
Phenotype
Antibodies
Antigens
Healthy Volunteers
Indirect Fluorescent Antibody Technique
Esophagus
Ulcer

Keywords

  • Antiepithelial cell antibodies
  • Chinese
  • HLA-DQ antigens
  • HLA-DR antigens
  • Recurrent oral ulcers

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Strong association of antiepithelial cell antibodies with HLA-DR3 or DR7 phenotype in patients with recurrent oral ulcers. / Sun, A.; Hsieh, R. P.; Liu, B. Y.; Wang, J. T.; Leu, J. S.; Wu, Y. C.; Chiang, C. P.

In: Journal of the Formosan Medical Association, Vol. 99, No. 4, 04.09.2000, p. 290-294.

Research output: Contribution to journalArticle

Sun, A. ; Hsieh, R. P. ; Liu, B. Y. ; Wang, J. T. ; Leu, J. S. ; Wu, Y. C. ; Chiang, C. P. / Strong association of antiepithelial cell antibodies with HLA-DR3 or DR7 phenotype in patients with recurrent oral ulcers. In: Journal of the Formosan Medical Association. 2000 ; Vol. 99, No. 4. pp. 290-294.
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T1 - Strong association of antiepithelial cell antibodies with HLA-DR3 or DR7 phenotype in patients with recurrent oral ulcers

AU - Sun, A.

AU - Hsieh, R. P.

AU - Liu, B. Y.

AU - Wang, J. T.

AU - Leu, J. S.

AU - Wu, Y. C.

AU - Chiang, C. P.

PY - 2000/9/4

Y1 - 2000/9/4

N2 - Background: Previous studies showed that antiepithelial cell antibodies (anti-ECA) were present in 71% (15/21) of patients with recurrent oral ulcers (ROU) and that there was a strong association of human leukocyte antigen (HLA)-DRw9 with ROU in Chinese patients. In this study, we assessed anti-ECA in a larger group of Chinese patients with ROU (n = 88) in order to further investigate the association of anti-ECA with HLA-DR and -DQ antigens. Methods: The anti-ECA in the sera of ROU patients were detected by an indirect immunofluorescence technique with rat esophagus as the substrate, and the HLA-DR and -DQ antigens in ROU patients were typed by a standard microcytotoxicity assay using Terasaki's oriental tray. Results: The rate of anti-ECA positivity was significantly higher (p < 0.0001) in ROU patients (68%) than in healthy control subjects (0%). Furthermore, the rate of anti-ECA positivity in patients with major or minor oral ulcers (72%) was significantly higher (29%) than that in patients with herpetiform ulcers (p < 0.05). There was a significant increase in the frequency of DR3 or DR7 antigen expression (p < 0.0001, p(c) [p corrected] < 0.001, relative risk [RR] = 4.3, etiologic fraction = 0.41) in anti-ECA-positive ROU patients compared with the corresponding frequencies in healthy control subjects. There was also a significant increase in the frequency of DR7 or DRw9 antigen expression (p < 0.005, p(c) < 0.05, RR = 4.7, etiologic fraction = 0.45) compared to healthy controls. Conclusions: Because only DR3 or DR7 antigen occurred more frequently in anti-ECA-positive than in anti-ECA-negative ROU patients (p < 0.0007, p(c) < 0.05, RR = 19.6, etiologic fraction = 0.51), we concluded that the gene coding for DR3 or DR7 antigen may contribute to the presence of anti-ECA in Chinese patients with ROU.

AB - Background: Previous studies showed that antiepithelial cell antibodies (anti-ECA) were present in 71% (15/21) of patients with recurrent oral ulcers (ROU) and that there was a strong association of human leukocyte antigen (HLA)-DRw9 with ROU in Chinese patients. In this study, we assessed anti-ECA in a larger group of Chinese patients with ROU (n = 88) in order to further investigate the association of anti-ECA with HLA-DR and -DQ antigens. Methods: The anti-ECA in the sera of ROU patients were detected by an indirect immunofluorescence technique with rat esophagus as the substrate, and the HLA-DR and -DQ antigens in ROU patients were typed by a standard microcytotoxicity assay using Terasaki's oriental tray. Results: The rate of anti-ECA positivity was significantly higher (p < 0.0001) in ROU patients (68%) than in healthy control subjects (0%). Furthermore, the rate of anti-ECA positivity in patients with major or minor oral ulcers (72%) was significantly higher (29%) than that in patients with herpetiform ulcers (p < 0.05). There was a significant increase in the frequency of DR3 or DR7 antigen expression (p < 0.0001, p(c) [p corrected] < 0.001, relative risk [RR] = 4.3, etiologic fraction = 0.41) in anti-ECA-positive ROU patients compared with the corresponding frequencies in healthy control subjects. There was also a significant increase in the frequency of DR7 or DRw9 antigen expression (p < 0.005, p(c) < 0.05, RR = 4.7, etiologic fraction = 0.45) compared to healthy controls. Conclusions: Because only DR3 or DR7 antigen occurred more frequently in anti-ECA-positive than in anti-ECA-negative ROU patients (p < 0.0007, p(c) < 0.05, RR = 19.6, etiologic fraction = 0.51), we concluded that the gene coding for DR3 or DR7 antigen may contribute to the presence of anti-ECA in Chinese patients with ROU.

KW - Antiepithelial cell antibodies

KW - Chinese

KW - HLA-DQ antigens

KW - HLA-DR antigens

KW - Recurrent oral ulcers

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M3 - Article

VL - 99

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EP - 294

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

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