Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis

Y. H. Luo, C. F. Kuo, K. J. Huang, J. J. Wu, H. Y. Lei, M. T. Lin, W. J. Chuang, C. C. Liu, C. F. Lin, Y. S. Lin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Streptococcal pyrogenic exotoxin B is an extracellular cysteine protease. Only nephritis-associated strains of group A streptococci secrete this protease and this may be involved in the pathogenesis of post-streptococcal glomerulonephritis. Mice were actively immunized with a recombinant protease inactive exotoxin B mutant or passively immunized with exotoxin B antibody. Characteristics of glomerulonephritis were measured using histology, immunoglobulin deposition, complement activation, cell infiltration, and proteinuria. None of the mice given bovine serum albumin or exotoxin A as controls showed any marked changes. Immunoglobulin deposition, complement activation, and leukocyte infiltration occurred only in the glomeruli of exotoxin B-hyperimmunized mice. One particular anti-exotoxin B monoclonal antibody, 10G, was cross-reactive with kidney endothelial cells and it caused kidney injury and proteinuria when infused into mice. This cross-reactivity may be involved in the pathogenesis of glomerulonephritis following group A streptococcal infection.

Original languageEnglish
Pages (from-to)716-724
Number of pages9
JournalKidney International
Volume72
Issue number6
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

Fingerprint

Exotoxins
Glomerulonephritis
Antibodies
Complement Activation
Proteinuria
Immunoglobulins
Peptide Hydrolases
Kidney
Streptococcal Infections
Cysteine Proteases
Nephritis
Bovine Serum Albumin
Streptococcus
Histology
Leukocytes
Endothelial Cells
Monoclonal Antibodies
erythrogenic toxin
Wounds and Injuries

Keywords

  • Anti-SPE B
  • Glomerulonephritis
  • Group A streptococcus
  • Mouse model

ASJC Scopus subject areas

  • Nephrology

Cite this

Luo, Y. H., Kuo, C. F., Huang, K. J., Wu, J. J., Lei, H. Y., Lin, M. T., ... Lin, Y. S. (2007). Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis. Kidney International, 72(6), 716-724. https://doi.org/10.1038/sj.ki.5002407

Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis. / Luo, Y. H.; Kuo, C. F.; Huang, K. J.; Wu, J. J.; Lei, H. Y.; Lin, M. T.; Chuang, W. J.; Liu, C. C.; Lin, C. F.; Lin, Y. S.

In: Kidney International, Vol. 72, No. 6, 09.2007, p. 716-724.

Research output: Contribution to journalArticle

Luo, YH, Kuo, CF, Huang, KJ, Wu, JJ, Lei, HY, Lin, MT, Chuang, WJ, Liu, CC, Lin, CF & Lin, YS 2007, 'Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis', Kidney International, vol. 72, no. 6, pp. 716-724. https://doi.org/10.1038/sj.ki.5002407
Luo, Y. H. ; Kuo, C. F. ; Huang, K. J. ; Wu, J. J. ; Lei, H. Y. ; Lin, M. T. ; Chuang, W. J. ; Liu, C. C. ; Lin, C. F. ; Lin, Y. S. / Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis. In: Kidney International. 2007 ; Vol. 72, No. 6. pp. 716-724.
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