Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis

Y. H. Luo, C. F. Kuo, K. J. Huang, J. J. Wu, H. Y. Lei, M. T. Lin, W. J. Chuang, C. C. Liu, C. F. Lin, Y. S. Lin

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Abstract

Streptococcal pyrogenic exotoxin B is an extracellular cysteine protease. Only nephritis-associated strains of group A streptococci secrete this protease and this may be involved in the pathogenesis of post-streptococcal glomerulonephritis. Mice were actively immunized with a recombinant protease inactive exotoxin B mutant or passively immunized with exotoxin B antibody. Characteristics of glomerulonephritis were measured using histology, immunoglobulin deposition, complement activation, cell infiltration, and proteinuria. None of the mice given bovine serum albumin or exotoxin A as controls showed any marked changes. Immunoglobulin deposition, complement activation, and leukocyte infiltration occurred only in the glomeruli of exotoxin B-hyperimmunized mice. One particular anti-exotoxin B monoclonal antibody, 10G, was cross-reactive with kidney endothelial cells and it caused kidney injury and proteinuria when infused into mice. This cross-reactivity may be involved in the pathogenesis of glomerulonephritis following group A streptococcal infection.

Original languageEnglish
Pages (from-to)716-724
Number of pages9
JournalKidney International
Volume72
Issue number6
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

Keywords

  • Anti-SPE B
  • Glomerulonephritis
  • Group A streptococcus
  • Mouse model

ASJC Scopus subject areas

  • Nephrology

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    Luo, Y. H., Kuo, C. F., Huang, K. J., Wu, J. J., Lei, H. Y., Lin, M. T., Chuang, W. J., Liu, C. C., Lin, C. F., & Lin, Y. S. (2007). Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis. Kidney International, 72(6), 716-724. https://doi.org/10.1038/sj.ki.5002407