Store-operated Ca2+ Entry Facilitates the Lipopolysaccharide-induced Cyclooxygenase-2 Expression in Gastric Cancer Cells

Jhen Hong Wong, Kuo Hao Ho, Sean Nam, Wen Li Hsu, Chia Hsien Lin, Che Mai Chang, Jaw Yuan Wang, Wei Chiao Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Helicobacter pylori has been identified as one of the major causes of chronic gastritis, gastric and duodenal ulcers, and gastric cancer. Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria, and H. pylori LPS might play an exclusively important role in activating inflammatory pathways in monocytes and macrophages. To study the role of LPS in the underlying mechanism of inflammatory responses, we established an in vitro model using the human AGS gastric cancer cell line. We found that LPS mediates inflammation through setting off a cascade of events: activation of the store-operated calcium (SOC) channel, initiation of downstream NF-κB signaling, and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). Phosphorylated ERK1/2 promotes the nuclear translocation of NF-κB, and eventually elevates the expression level of COX-2, a major inflammatory gene.

Original languageEnglish
Article number21813
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Cyclooxygenase 2
Stomach Neoplasms
Lipopolysaccharides
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Duodenal Neoplasms
Gastritis
Stomach Ulcer
Calcium Channels
Duodenal Ulcer
Gram-Negative Bacteria
Helicobacter pylori
Monocytes
Macrophages
Phosphorylation
Inflammation
Cell Line
Membranes
Genes

ASJC Scopus subject areas

  • General

Cite this

Store-operated Ca2+ Entry Facilitates the Lipopolysaccharide-induced Cyclooxygenase-2 Expression in Gastric Cancer Cells. / Wong, Jhen Hong; Ho, Kuo Hao; Nam, Sean; Hsu, Wen Li; Lin, Chia Hsien; Chang, Che Mai; Wang, Jaw Yuan; Chang, Wei Chiao.

In: Scientific Reports, Vol. 7, No. 1, 21813, 01.12.2017.

Research output: Contribution to journalArticle

Wong, Jhen Hong ; Ho, Kuo Hao ; Nam, Sean ; Hsu, Wen Li ; Lin, Chia Hsien ; Chang, Che Mai ; Wang, Jaw Yuan ; Chang, Wei Chiao. / Store-operated Ca2+ Entry Facilitates the Lipopolysaccharide-induced Cyclooxygenase-2 Expression in Gastric Cancer Cells. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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AU - Lin, Chia Hsien

AU - Chang, Che Mai

AU - Wang, Jaw Yuan

AU - Chang, Wei Chiao

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