Stimuli-responsive materials prepared from carboxymethyl chitosan and poly(γ-glutamic acid) for protein delivery

Shu Huei Yu, Shao Jung Wu, Deh Wei Tang, Yi Cheng Ho, Fwu Long Mi, Tzu Hung Kuo, Hsing Wen Sung

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


The development of stimuli-responsive materials in response to the molecules involved in biological processes has gained increased attentions. In this work, carboxymethyl chitosan (CM-chitosan) and poly(γ-glutamic acid) (pGlu) were reacted with a naturally occurring compound, genipin, leading to the formation of genipin-crosslinked CM-chitosan/pGlu conjugates with fluorescence emissions. The genipin-conjugated polymers were sensitive to the oxidation product of glucose, gluconic acid and hydrogen peroxide (H 2O 2). Fluorescence emissions of the polymers were quenched by gluconic acid and H 2O 2. An increase in the hydrodynamic diameter together with the quenching of fluorescence indicated that the genipin-conjugated polymers were self-aggregated into nanoparticles, in response to the stimulus of gluconic acid (but not for H 2O 2). Bovine serum albumin (BSA) could be loaded in the self-aggregated nanoparticles, and the incorporated BSA slowly released from the nanoparticles under hyper-gluconic acid conditions. This material is hence proposed as a stimuli-responsive material for optical sensing and protein delivery purposes.

Original languageEnglish
Pages (from-to)531-536
Number of pages6
JournalCarbohydrate Polymers
Issue number1
Publication statusPublished - Jan 4 2012
Externally publishedYes


  • Chitosan
  • Genipin
  • Protein delivery
  • Stimuli-responsive

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

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