Abstract
The effect of dopamine (DA) on acid secretion was studied using the everted preparation of isolated rat stomachs. DA concentrations, measured by HPLC-ECD in the rumen, corpus and antrum were 1.06 nmol/mg protein, 0.49 nmol/mg protein and 2.92 nmol/mg protein, respectively. DA stimulated acid secretion at a concentration of 10 nM and elicited the maximum response at 10 μM, which was at a level approximately 1.56-fold that of the spontaneous secretion but only about half that of secretion induced by histamine at a concentration of 0.3 mM. The concentration-dependent stimulation by DA was antagonized by octopamine and SCH 23390. Failure of proglumide and cimetidine to affect this stimulation ruled out the participation of histamine and/or gastrin. Scopolamine and tetrodotoxin completely inhibited the acid secretion induced by low concentrations of DA but inhibited only partially the response induced by high concentrations of DA. The results obtained indicate that DA induces acid secretion via activation of the dopamine D1 receptor, located on the cholinergic neurons and on some nonneuronal cells, in the rat stomach.
| Original language | English |
|---|---|
| Pages (from-to) | 235-240 |
| Number of pages | 6 |
| Journal | Neuroscience Research |
| Volume | 21 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1995 |
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Keywords
- Acid secretion
- Cholinergic neuron
- Cimetidine
- Dopamine
- Isolated rat stomach
- Octopamine
- Proglumide
- SCH 23390
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Stimulatory effect of dopamine on acid secretion from the isolated rat stomach. / Tsai, Li Hsueh; Cheng, Juei Tang.
In: Neuroscience Research, Vol. 21, No. 3, 1995, p. 235-240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stimulatory effect of dopamine on acid secretion from the isolated rat stomach
AU - Tsai, Li Hsueh
AU - Cheng, Juei Tang
PY - 1995
Y1 - 1995
N2 - The effect of dopamine (DA) on acid secretion was studied using the everted preparation of isolated rat stomachs. DA concentrations, measured by HPLC-ECD in the rumen, corpus and antrum were 1.06 nmol/mg protein, 0.49 nmol/mg protein and 2.92 nmol/mg protein, respectively. DA stimulated acid secretion at a concentration of 10 nM and elicited the maximum response at 10 μM, which was at a level approximately 1.56-fold that of the spontaneous secretion but only about half that of secretion induced by histamine at a concentration of 0.3 mM. The concentration-dependent stimulation by DA was antagonized by octopamine and SCH 23390. Failure of proglumide and cimetidine to affect this stimulation ruled out the participation of histamine and/or gastrin. Scopolamine and tetrodotoxin completely inhibited the acid secretion induced by low concentrations of DA but inhibited only partially the response induced by high concentrations of DA. The results obtained indicate that DA induces acid secretion via activation of the dopamine D1 receptor, located on the cholinergic neurons and on some nonneuronal cells, in the rat stomach.
AB - The effect of dopamine (DA) on acid secretion was studied using the everted preparation of isolated rat stomachs. DA concentrations, measured by HPLC-ECD in the rumen, corpus and antrum were 1.06 nmol/mg protein, 0.49 nmol/mg protein and 2.92 nmol/mg protein, respectively. DA stimulated acid secretion at a concentration of 10 nM and elicited the maximum response at 10 μM, which was at a level approximately 1.56-fold that of the spontaneous secretion but only about half that of secretion induced by histamine at a concentration of 0.3 mM. The concentration-dependent stimulation by DA was antagonized by octopamine and SCH 23390. Failure of proglumide and cimetidine to affect this stimulation ruled out the participation of histamine and/or gastrin. Scopolamine and tetrodotoxin completely inhibited the acid secretion induced by low concentrations of DA but inhibited only partially the response induced by high concentrations of DA. The results obtained indicate that DA induces acid secretion via activation of the dopamine D1 receptor, located on the cholinergic neurons and on some nonneuronal cells, in the rat stomach.
KW - Acid secretion
KW - Cholinergic neuron
KW - Cimetidine
KW - Dopamine
KW - Isolated rat stomach
KW - Octopamine
KW - Proglumide
KW - SCH 23390
UR - http://www.scopus.com/inward/record.url?scp=0028940874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028940874&partnerID=8YFLogxK
U2 - 10.1016/0168-0102(94)00854-9
DO - 10.1016/0168-0102(94)00854-9
M3 - Article
C2 - 7753504
AN - SCOPUS:0028940874
VL - 21
SP - 235
EP - 240
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 3
ER -