Stimulatory effect of D-ephedrine on β3-adrenoceptors in adipose tissue of rats

Juei Tang Cheng, I. Min Liu, Shi Ting Yen, Shiow Wen Juang, Tsang Pai Liu, Paul Chan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of ephedrine on β3-adrenoceptors (β3-AR) was studied in the isolated adipose tissue of Wistar rat. Incubation with D-ephedrine (0.1-10 μM) induced a concentration-dependent decrease of uptake of [14C]-deoxy-D-glucose into white adipose tissues (WAT). The inhibitory effect of D-ephedrine was potentiated by BRL 37344, the agonist of β3-AR and concentration-dependently inhibited by SR 59230A, the selective antagonist of β3-AR. The action of D-ephedrine on β3-AR was further blocked by the antibodies for β3-AR, but not the immunoglobulin, in a concentration-dependent manner. Moreover, D-ephedrine increased glycerol release from the isolated brown adipose tissues (BAT) and this action was also abolished by SR 59230A at concentration sufficient to block β3-AR. Thus, these results suggest that D-ephedrine has the ability to activate β3-AR both in WAT and BAT of Wistar rats in vitro.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalAutonomic Neuroscience: Basic and Clinical
Volume88
Issue number1-2
DOIs
Publication statusPublished - Apr 12 2001

Keywords

  • β-Adrenoceptors
  • BRL 37344
  • Brown adipose tissues
  • D-Ephedrine
  • Glucose uptake
  • Lipolysis
  • SR 59230A
  • White adipose tissues

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems

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