Stimulative effect of prostaglandins on production of hexosamine-containing substances by cultured fibroblasts (2) early effect of various prostaglandins at various doses

Sei itsu Murota, Midori Abe, Katsuhiro Otsuka, Wen Chang Chang

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Abstract

Early effects of various prostaglandins on the production of hexosamine-containing substances by cultured fibroblasts, which were derived from a rat carrageenin granuloma, were studied. At the stationary phase, the cells were exposed for 6 h to one of the prostaglandin A1 (PGA1), A2, B1, B2, D2, F, F, E1, E2 or arachidonic acid in various concentrations ranging from 0.01 to 10 μg/ml for all the stimuli and from 10 pg to 10 μg/ml for PGF. The activity of the cells in incorporating 3H-glucosamine into hexosamine-containing substances (acidic) glycosaminoglycans and glycoproteins) during this period was compared with that of control cells. All the stimuli tested showed more or less stimulative effect on the synthesis of hexosamine-containing substances at their specific concentrations. PGF was found to be the most potent stimulant and its stimulative effect was found significant even at the low concentration of 100 pg/ml. PGD2, F and E2 were the next potent stimuli. Their optimum dose were around 1 μg/ml but they still had significant stimulation at the concentration of 0.01 μg/ml. Effect of PGE2 was rather mild. Stimulation by PGA1, A2, B1 and B2 or arachidonic acid was seen at high dose, and its seemed to be non-specific. The results suggested that these prostaglandins such as PGF, D2, F and E2 play some important role on regulating the production of intercellular ground substances.

Original languageEnglish
Pages (from-to)711-717
Number of pages7
JournalProstaglandins
Volume13
Issue number4
DOIs
Publication statusPublished - 1977
Externally publishedYes

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Hexosamines
Dinoprost
Fibroblasts
Prostaglandins
Arachidonic Acid
Prostaglandin D2
Carrageenan
Glucosamine
Glycosaminoglycans
Granuloma
Dinoprostone
Rats
Glycoproteins
Cells
prostaglandin A1
prostaglandin A2

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Stimulative effect of prostaglandins on production of hexosamine-containing substances by cultured fibroblasts (2) early effect of various prostaglandins at various doses. / Murota, Sei itsu; Abe, Midori; Otsuka, Katsuhiro; Chang, Wen Chang.

In: Prostaglandins, Vol. 13, No. 4, 1977, p. 711-717.

Research output: Contribution to journalArticle

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