Stimulation of pulmonary vagal C-fibres by anandamide in anaesthetized rats: Role of vanilloid type 1 receptors

You Shuei Lin, Lu Yuan Lee

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

This study was carried out to determine the effect of intravenous injection of anandamide on pulmonary C-fibre afferents and the cardiorespiratory reflexes. In anaesthetized, spontaneously breathing rats, intravenous bolus injection of anandamide near the right atrium immediately elicited the pulmonary chemoreflex responses, characterized by apnoea, bradycardia and hypotension. After perineural treatment of both cervical vagi with capsaicin to block the conduction of C-fibres, anandamide no longer evoked these reflex responses. In open-chest, and artificially ventilated rats, anandamide injection evoked an abrupt and intense discharge in vagal pulmonary C-fibres in a dose-dependent manner. After injection of the high dose, the fibre discharge generally started within 1 s, reached a peak in -2 s, and returned to baseline within 7 s. The stimulation of C-fibres by anandamide was completely and reversibly blocked by pretreatment with capsazepine, a competitive antagonist of the vanilloid type 1 receptor. Anandamide (0.4 mg kg-1) stimulated ∼93 % of pulmonary C-fibres that were activated by capsaicin at a much lower dose (0.6 μg kg-1); the response to anandamide showed similar intensity, but had slightly longer latency and duration than that to capsaicin. In conclusion, intravenous bolus injection of anandamide evokes a consistent and distinct stimulatory effect on pulmonary C-fibre terminals, and this effect appears to be mediated through an activation of the vanilloid type I receptor.

Original languageEnglish
Pages (from-to)947-955
Number of pages9
JournalJournal of Physiology
Volume539
Issue number3
DOIs
Publication statusPublished - Mar 15 2002
Externally publishedYes

Fingerprint

Unmyelinated Nerve Fibers
Lung
Capsaicin
Intravenous Injections
Reflex
TRPV Cation Channels
Injections
anandamide
rat Trpv1 protein
Apnea
Bradycardia
Heart Atria
Hypotension
Respiration
Thorax

ASJC Scopus subject areas

  • Physiology

Cite this

Stimulation of pulmonary vagal C-fibres by anandamide in anaesthetized rats : Role of vanilloid type 1 receptors. / Lin, You Shuei; Lee, Lu Yuan.

In: Journal of Physiology, Vol. 539, No. 3, 15.03.2002, p. 947-955.

Research output: Contribution to journalArticle

@article{dee76958e124444289cd6db2de541d21,
title = "Stimulation of pulmonary vagal C-fibres by anandamide in anaesthetized rats: Role of vanilloid type 1 receptors",
abstract = "This study was carried out to determine the effect of intravenous injection of anandamide on pulmonary C-fibre afferents and the cardiorespiratory reflexes. In anaesthetized, spontaneously breathing rats, intravenous bolus injection of anandamide near the right atrium immediately elicited the pulmonary chemoreflex responses, characterized by apnoea, bradycardia and hypotension. After perineural treatment of both cervical vagi with capsaicin to block the conduction of C-fibres, anandamide no longer evoked these reflex responses. In open-chest, and artificially ventilated rats, anandamide injection evoked an abrupt and intense discharge in vagal pulmonary C-fibres in a dose-dependent manner. After injection of the high dose, the fibre discharge generally started within 1 s, reached a peak in -2 s, and returned to baseline within 7 s. The stimulation of C-fibres by anandamide was completely and reversibly blocked by pretreatment with capsazepine, a competitive antagonist of the vanilloid type 1 receptor. Anandamide (0.4 mg kg-1) stimulated ∼93 {\%} of pulmonary C-fibres that were activated by capsaicin at a much lower dose (0.6 μg kg-1); the response to anandamide showed similar intensity, but had slightly longer latency and duration than that to capsaicin. In conclusion, intravenous bolus injection of anandamide evokes a consistent and distinct stimulatory effect on pulmonary C-fibre terminals, and this effect appears to be mediated through an activation of the vanilloid type I receptor.",
author = "Lin, {You Shuei} and Lee, {Lu Yuan}",
year = "2002",
month = "3",
day = "15",
doi = "10.1113/jphysiol.2001.013290",
language = "English",
volume = "539",
pages = "947--955",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Stimulation of pulmonary vagal C-fibres by anandamide in anaesthetized rats

T2 - Role of vanilloid type 1 receptors

AU - Lin, You Shuei

AU - Lee, Lu Yuan

PY - 2002/3/15

Y1 - 2002/3/15

N2 - This study was carried out to determine the effect of intravenous injection of anandamide on pulmonary C-fibre afferents and the cardiorespiratory reflexes. In anaesthetized, spontaneously breathing rats, intravenous bolus injection of anandamide near the right atrium immediately elicited the pulmonary chemoreflex responses, characterized by apnoea, bradycardia and hypotension. After perineural treatment of both cervical vagi with capsaicin to block the conduction of C-fibres, anandamide no longer evoked these reflex responses. In open-chest, and artificially ventilated rats, anandamide injection evoked an abrupt and intense discharge in vagal pulmonary C-fibres in a dose-dependent manner. After injection of the high dose, the fibre discharge generally started within 1 s, reached a peak in -2 s, and returned to baseline within 7 s. The stimulation of C-fibres by anandamide was completely and reversibly blocked by pretreatment with capsazepine, a competitive antagonist of the vanilloid type 1 receptor. Anandamide (0.4 mg kg-1) stimulated ∼93 % of pulmonary C-fibres that were activated by capsaicin at a much lower dose (0.6 μg kg-1); the response to anandamide showed similar intensity, but had slightly longer latency and duration than that to capsaicin. In conclusion, intravenous bolus injection of anandamide evokes a consistent and distinct stimulatory effect on pulmonary C-fibre terminals, and this effect appears to be mediated through an activation of the vanilloid type I receptor.

AB - This study was carried out to determine the effect of intravenous injection of anandamide on pulmonary C-fibre afferents and the cardiorespiratory reflexes. In anaesthetized, spontaneously breathing rats, intravenous bolus injection of anandamide near the right atrium immediately elicited the pulmonary chemoreflex responses, characterized by apnoea, bradycardia and hypotension. After perineural treatment of both cervical vagi with capsaicin to block the conduction of C-fibres, anandamide no longer evoked these reflex responses. In open-chest, and artificially ventilated rats, anandamide injection evoked an abrupt and intense discharge in vagal pulmonary C-fibres in a dose-dependent manner. After injection of the high dose, the fibre discharge generally started within 1 s, reached a peak in -2 s, and returned to baseline within 7 s. The stimulation of C-fibres by anandamide was completely and reversibly blocked by pretreatment with capsazepine, a competitive antagonist of the vanilloid type 1 receptor. Anandamide (0.4 mg kg-1) stimulated ∼93 % of pulmonary C-fibres that were activated by capsaicin at a much lower dose (0.6 μg kg-1); the response to anandamide showed similar intensity, but had slightly longer latency and duration than that to capsaicin. In conclusion, intravenous bolus injection of anandamide evokes a consistent and distinct stimulatory effect on pulmonary C-fibre terminals, and this effect appears to be mediated through an activation of the vanilloid type I receptor.

UR - http://www.scopus.com/inward/record.url?scp=0037088844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037088844&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2001.013290

DO - 10.1113/jphysiol.2001.013290

M3 - Article

C2 - 11897863

AN - SCOPUS:0037088844

VL - 539

SP - 947

EP - 955

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 3

ER -