The effect of estradiol administration on renal prostaglandin (PG) E2 biosynthetic activity in rats was studied. A specific radioimmunoassay for PGE2 was developed and applied in the quantitation of PGE2 biosynthesis in kidney. Conversion of exogenous arachidonic acid into PGE2 by renal microsomal fraction was assayed. Formation of PGE2 was linear in fashion up to 5 min incubation at 37°C, and linear in fashion up to 3.5 mg of microsome used as enzyme source. The renal biosynthesis of PGE2 was significantly increased by estradiol treatment.
|Number of pages||5|
|Journal||Prostaglandins Leukotrienes and Essential Fatty Acids|
|Publication status||Published - 1988|
ASJC Scopus subject areas
- Clinical Biochemistry
- Endocrinology, Diabetes and Metabolism