Stimulation of osteogenic activity in human osteoblast cells by edible Uraria crinita

Yi Wen Mao, Rong Dih Lin, Hsiao Chiao Hung, Mei Hsien Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Uraria crinita is an edible herb used as a natural food for childhood skeletal dysplasia. Ethyl acetate, n-butanol, and aqueous fractions of a 95% ethanol crude extract of U. crinita were obtained and the active ingredients isolated and purified using a bioguided method. In this manner, we isolated and identified a new active flavone glycoside, apigenin 6-C-β-d- apiofuranosyl(1→2)-α-d-xylopyranoside (3) and 10 known components with stimulatory activity on human osteoblast cells. The new compound 3 at 100 μM significantly increased alkaline phosphatase activity (114.10 ± 4.41%), mineralization (150.10 ± 0.80%), as well as osteopontin (1.39 ± 0.01-fold), bone morphogenetic protein-2 (BMP-2, 1.30 ± 0.04-fold), and runt-related transcription factor 2 (Runx2, 1.43 ± 0.10-fold) mRNA expression through the activation of the BMP-2/Runx2 pathway. Two other components, dalbergioidin (1) and byzantionoside B (9), displayed similar effects. These results show that U. crinita and its active compounds may have the potential to stimulate bone formation and regeneration.

Original languageEnglish
Pages (from-to)5581-5588
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number24
DOIs
Publication statusPublished - Jun 18 2014

Keywords

  • alkaline phosphatase
  • apigenin 6- C -β- d -apiofuranosyl(1→2)-α- d -xylopyranoside
  • BMP-2/Runx2 pathway
  • human osteoblast cells
  • mineralization
  • osteopontin
  • Uraria crinita

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

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