Stimulation of Early Osteochondral Differentiation of Human Mesenchymal Stem Cells Using Binary Colloidal Crystals (BCCs)

Peng Yuan Wang, Helmut Thissen, Peter Kingshott

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A new surface based on self-assembly of two colloids into well-defined nanostructures, so-called binary colloidal crystals (BCCs), was fabricated for stem cell culture. The facile fabrication process are able to cover large surface areas (>3 cm-diameter, i.e. > 7 cm2) with ordered surface nanotopographies that is often a challenge particularly in biomaterials science. From our library, four different combinations of BCCs were selected using mixtures of silica, polystyrene and poly(methyl methacrylate) particles with sizes in the range from 100 nm to 5 μm. Cell spreading, proliferation, and surface-induced lineage commitment of human adipose-derived stem cells (hADSCs) was studied using quantitative real time polymerase chain reaction (qRT-PCR) and immunostaining. The results showed that BCCs induced osteo- and chondro- but not adipo-gene expression in the absence of induction medium suggesting that the osteochondral lineage can be stimulated by the BCCs. When applying induction media, higher osteo- and chondro-gene expression on BCCs was found compared with tissue culture polystyrene (TCPS) and flat silica (Si) controls, respectively. Colony forming of chondrogenic hADSCs was found on BCCs and TCPS but not Si controls, suggesting that the differentiation of stem cells is surface-dependent. BCCs provide access to complex nanotopographies and chemistries, which can find applications in cell culture and regenerative medicine.

Original languageEnglish
Pages (from-to)4477-4488
Number of pages12
JournalACS Applied Materials and Interfaces
Volume8
Issue number7
DOIs
Publication statusPublished - Feb 24 2016
Externally publishedYes

Fingerprint

Stem cells
Crystals
Polystyrenes
Silicon Dioxide
Tissue culture
Silica
Cell culture
Gene expression
Polymerase chain reaction
Cell proliferation
Colloids
Biocompatible Materials
Polymethyl Methacrylate
Polymethyl methacrylates
Biomaterials
Self assembly
Nanostructures
Genes
Fabrication

Keywords

  • adipogenesis
  • binary colloidal crystals
  • chondrogenesis
  • human stem cells
  • osteogenesis

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Stimulation of Early Osteochondral Differentiation of Human Mesenchymal Stem Cells Using Binary Colloidal Crystals (BCCs). / Wang, Peng Yuan; Thissen, Helmut; Kingshott, Peter.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 7, 24.02.2016, p. 4477-4488.

Research output: Contribution to journalArticle

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