The effects of estradiol on the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and the 12-lipoxygenase activity in rat platelets in vivo were studied. Estradiol had no significant effect on the endogenous fatty acid composition of cellular phospholipid fractions. However, estradiol significantly increased 12-lipoxygenase activity in platelets in a dose-dependent manner. The stimulatory effect of estradiol on platelet lipoxygenase was blocked by the anti-estrogen nafoxidine hydrochloride which was injected simultaneously together with estradiol in vivo, suggesting that the effect in target cells was due directly to estradiol.
- 12-l-hydoxy-5,8,10,14-eicosatetraenoic acid
- 12-l-hydroperoxy-5,8,10,14-eicosatetraenoic acid
- 12-l-hydroxy-5,8,10-heptadecatrienoic acid
- and GLC
- gas-liquid chromtography
- reduced glugatione
ASJC Scopus subject areas