STIM1- and Orai1-mediated Ca2+ oscillation orchestrates invadopodium formation and melanoma invasion

Jianwei Sun, Fujian Lu, Huifang He, Junling Shen, Jane Messina, Rahel Mathew, Dapeng Wang, Amod A. Sarnaik, Wei Chiao Chang, Minjung Kim, Heping Cheng, Shengyu Yang

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Abstract

Ca2+ signaling has been increasingly implicated in cancer invasion and metastasis, and yet, the underlying mechanisms remained largely unknown. In this paper, we report that STIM1- and Orai1-mediated Ca2+ oscillations promote melanoma invasion by orchestrating invadopodium assembly and extracellular matrix (ECM) degradation. Ca2+ oscillation signals facilitate invadopodial precursor assembly by activating Src. Disruption of Ca2+ oscillations inhibited invadopodium assembly. Furthermore, STIM1 and Orai1 regulate the proteolysis activity of individual invadopodia. Mechanistically, Orai1 blockade inhibited the recycling of MT1-matrix metalloproteinase (MMP) to the plasma membrane and entrapped MT1-MMP in the endocytic compartment to inhibit ECM degradation. STIM1 knockdown significantly inhibited melanoma lung metastasis in a xenograft mouse model, implicating the importance of this pathway in metastatic dissemination. Our findings provide a novel mechanism for Ca2+-mediated cancer cell invasion and shed new light on the spatiotemporal organization of store-operated Ca2+ signals during melanoma invasion and metastasis.

Original languageEnglish
Pages (from-to)535-548
Number of pages14
JournalJournal of Cell Biology
Volume207
Issue number4
DOIs
Publication statusPublished - 2014

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Matrix Metalloproteinase 14
Melanoma
Neoplasm Metastasis
Extracellular Matrix
Recycling
Heterografts
Proteolysis
Neoplasms
Cell Membrane
Lung
Podosomes

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

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STIM1- and Orai1-mediated Ca2+ oscillation orchestrates invadopodium formation and melanoma invasion. / Sun, Jianwei; Lu, Fujian; He, Huifang; Shen, Junling; Messina, Jane; Mathew, Rahel; Wang, Dapeng; Sarnaik, Amod A.; Chang, Wei Chiao; Kim, Minjung; Cheng, Heping; Yang, Shengyu.

In: Journal of Cell Biology, Vol. 207, No. 4, 2014, p. 535-548.

Research output: Contribution to journalArticle

Sun, J, Lu, F, He, H, Shen, J, Messina, J, Mathew, R, Wang, D, Sarnaik, AA, Chang, WC, Kim, M, Cheng, H & Yang, S 2014, 'STIM1- and Orai1-mediated Ca2+ oscillation orchestrates invadopodium formation and melanoma invasion', Journal of Cell Biology, vol. 207, no. 4, pp. 535-548. https://doi.org/10.1083/jcb.201407082
Sun, Jianwei ; Lu, Fujian ; He, Huifang ; Shen, Junling ; Messina, Jane ; Mathew, Rahel ; Wang, Dapeng ; Sarnaik, Amod A. ; Chang, Wei Chiao ; Kim, Minjung ; Cheng, Heping ; Yang, Shengyu. / STIM1- and Orai1-mediated Ca2+ oscillation orchestrates invadopodium formation and melanoma invasion. In: Journal of Cell Biology. 2014 ; Vol. 207, No. 4. pp. 535-548.
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