Steroids and antihistamines synergize to inhibit rat’s airway smooth muscle contractility

Shao Cheng Liu, Yueng Hsiang Chu, Chuan Hsiang Kao, Chi Chung Wu, Hsing Won Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Both glucocorticoids and H1-antihistamines were widely used on patients with allergic rhinitis (AR) and obstructive airway diseases. However, their direct effects on airway smooth muscle were not fully explored. In this study, we tested the effectiveness of prednisolone (Kidsolone) and levocetirizine (Xyzal) on isolated rat trachea submersed in Kreb’s solution in a muscle bath. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured. The following assessments of the drug were performed: (1) effect on tracheal smooth muscle resting tension; (2) effect on contraction caused by 10−6 M methacholine; (3) effect of the drug on electrical field stimulation (EFS) induced tracheal smooth muscle contractions. The result revealed sole use of Kidsolone or Xyzal elicited no significant effect or only a little relaxation response on tracheal tension after methacholine treatment. The tension was 90.5 ± 7.5 and 99.5 ± 0.8 % at 10−4 M for Xyzal and 10−5 M for Kidsolone, respectively. However, a dramatically spasmolytic effect was observed after co-administration of Kidsolone and Xyzal and the tension dropped to 67.5 ± 13.6 %, with statistical significance (p <0.05). As for EFS-induced contractions, Kidsolone had no direct effect but Xyzal could inhibit it, with increasing basal tension. In conclusion, using glucocorticoids alone had no spasmolytic effect but they can be synergized with antihistamines to dramatically relax the trachea smooth muscle within minutes. Therefore, for AR patients with acute asthma attack, combined use of those two drugs is recommended.

Original languageEnglish
Pages (from-to)1443-1449
Number of pages7
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume272
Issue number6
DOIs
Publication statusPublished - Jun 28 2015

Fingerprint

Histamine Antagonists
Smooth Muscle
Steroids
Parasympatholytics
Methacholine Chloride
Trachea
Glucocorticoids
Electric Stimulation
Pharmaceutical Preparations
Muscle Tonus
Muscle Contraction
Prednisolone
Baths
levocetirizine
Asthma
Muscles

Keywords

  • Antihistamine
  • Cholinergic effect
  • Muscarinic
  • Steroid
  • Trachea smooth muscle

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Steroids and antihistamines synergize to inhibit rat’s airway smooth muscle contractility. / Liu, Shao Cheng; Chu, Yueng Hsiang; Kao, Chuan Hsiang; Wu, Chi Chung; Wang, Hsing Won.

In: European Archives of Oto-Rhino-Laryngology, Vol. 272, No. 6, 28.06.2015, p. 1443-1449.

Research output: Contribution to journalArticle

Liu, Shao Cheng ; Chu, Yueng Hsiang ; Kao, Chuan Hsiang ; Wu, Chi Chung ; Wang, Hsing Won. / Steroids and antihistamines synergize to inhibit rat’s airway smooth muscle contractility. In: European Archives of Oto-Rhino-Laryngology. 2015 ; Vol. 272, No. 6. pp. 1443-1449.
@article{ab6f329feb0e41b298cd75b37dc07cbf,
title = "Steroids and antihistamines synergize to inhibit rat’s airway smooth muscle contractility",
abstract = "Both glucocorticoids and H1-antihistamines were widely used on patients with allergic rhinitis (AR) and obstructive airway diseases. However, their direct effects on airway smooth muscle were not fully explored. In this study, we tested the effectiveness of prednisolone (Kidsolone) and levocetirizine (Xyzal) on isolated rat trachea submersed in Kreb’s solution in a muscle bath. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured. The following assessments of the drug were performed: (1) effect on tracheal smooth muscle resting tension; (2) effect on contraction caused by 10−6 M methacholine; (3) effect of the drug on electrical field stimulation (EFS) induced tracheal smooth muscle contractions. The result revealed sole use of Kidsolone or Xyzal elicited no significant effect or only a little relaxation response on tracheal tension after methacholine treatment. The tension was 90.5 ± 7.5 and 99.5 ± 0.8 {\%} at 10−4 M for Xyzal and 10−5 M for Kidsolone, respectively. However, a dramatically spasmolytic effect was observed after co-administration of Kidsolone and Xyzal and the tension dropped to 67.5 ± 13.6 {\%}, with statistical significance (p <0.05). As for EFS-induced contractions, Kidsolone had no direct effect but Xyzal could inhibit it, with increasing basal tension. In conclusion, using glucocorticoids alone had no spasmolytic effect but they can be synergized with antihistamines to dramatically relax the trachea smooth muscle within minutes. Therefore, for AR patients with acute asthma attack, combined use of those two drugs is recommended.",
keywords = "Antihistamine, Cholinergic effect, Muscarinic, Steroid, Trachea smooth muscle",
author = "Liu, {Shao Cheng} and Chu, {Yueng Hsiang} and Kao, {Chuan Hsiang} and Wu, {Chi Chung} and Wang, {Hsing Won}",
year = "2015",
month = "6",
day = "28",
doi = "10.1007/s00405-014-3240-y",
language = "English",
volume = "272",
pages = "1443--1449",
journal = "European Archives of Oto-Rhino-Laryngology",
issn = "0937-4477",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Steroids and antihistamines synergize to inhibit rat’s airway smooth muscle contractility

AU - Liu, Shao Cheng

AU - Chu, Yueng Hsiang

AU - Kao, Chuan Hsiang

AU - Wu, Chi Chung

AU - Wang, Hsing Won

PY - 2015/6/28

Y1 - 2015/6/28

N2 - Both glucocorticoids and H1-antihistamines were widely used on patients with allergic rhinitis (AR) and obstructive airway diseases. However, their direct effects on airway smooth muscle were not fully explored. In this study, we tested the effectiveness of prednisolone (Kidsolone) and levocetirizine (Xyzal) on isolated rat trachea submersed in Kreb’s solution in a muscle bath. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured. The following assessments of the drug were performed: (1) effect on tracheal smooth muscle resting tension; (2) effect on contraction caused by 10−6 M methacholine; (3) effect of the drug on electrical field stimulation (EFS) induced tracheal smooth muscle contractions. The result revealed sole use of Kidsolone or Xyzal elicited no significant effect or only a little relaxation response on tracheal tension after methacholine treatment. The tension was 90.5 ± 7.5 and 99.5 ± 0.8 % at 10−4 M for Xyzal and 10−5 M for Kidsolone, respectively. However, a dramatically spasmolytic effect was observed after co-administration of Kidsolone and Xyzal and the tension dropped to 67.5 ± 13.6 %, with statistical significance (p <0.05). As for EFS-induced contractions, Kidsolone had no direct effect but Xyzal could inhibit it, with increasing basal tension. In conclusion, using glucocorticoids alone had no spasmolytic effect but they can be synergized with antihistamines to dramatically relax the trachea smooth muscle within minutes. Therefore, for AR patients with acute asthma attack, combined use of those two drugs is recommended.

AB - Both glucocorticoids and H1-antihistamines were widely used on patients with allergic rhinitis (AR) and obstructive airway diseases. However, their direct effects on airway smooth muscle were not fully explored. In this study, we tested the effectiveness of prednisolone (Kidsolone) and levocetirizine (Xyzal) on isolated rat trachea submersed in Kreb’s solution in a muscle bath. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured. The following assessments of the drug were performed: (1) effect on tracheal smooth muscle resting tension; (2) effect on contraction caused by 10−6 M methacholine; (3) effect of the drug on electrical field stimulation (EFS) induced tracheal smooth muscle contractions. The result revealed sole use of Kidsolone or Xyzal elicited no significant effect or only a little relaxation response on tracheal tension after methacholine treatment. The tension was 90.5 ± 7.5 and 99.5 ± 0.8 % at 10−4 M for Xyzal and 10−5 M for Kidsolone, respectively. However, a dramatically spasmolytic effect was observed after co-administration of Kidsolone and Xyzal and the tension dropped to 67.5 ± 13.6 %, with statistical significance (p <0.05). As for EFS-induced contractions, Kidsolone had no direct effect but Xyzal could inhibit it, with increasing basal tension. In conclusion, using glucocorticoids alone had no spasmolytic effect but they can be synergized with antihistamines to dramatically relax the trachea smooth muscle within minutes. Therefore, for AR patients with acute asthma attack, combined use of those two drugs is recommended.

KW - Antihistamine

KW - Cholinergic effect

KW - Muscarinic

KW - Steroid

KW - Trachea smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=84938225936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938225936&partnerID=8YFLogxK

U2 - 10.1007/s00405-014-3240-y

DO - 10.1007/s00405-014-3240-y

M3 - Article

VL - 272

SP - 1443

EP - 1449

JO - European Archives of Oto-Rhino-Laryngology

JF - European Archives of Oto-Rhino-Laryngology

SN - 0937-4477

IS - 6

ER -