Staurosporine modulates radiosensitivity and radiation-induced apoptosis in U937 cells

How Ran Guo, Chia Hsin Chen, Sheng Yow Ho, Yuan Soon Ho, Rong Jane Chen, Ying Jan Wang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: The present study aims at investigating the involvement of several genes in the cell cycle distribution and apoptosis in U937 cells, a cell line lacking functional p53 protein, after combined treatment with staurosporine and irradiation. Materials and methods: Using a DNA fragmentation assay, flow cytometry and western blot analysis, the molecular basis for the effects of staurosporine in combination with the irradiation of leukemia cells was investigated. Results: Our results indicated that combined treatment led to an increased apoptotic cell death in U937 cells, which is correlated with the phosphorylation of the V-Jun sarcoma virus 17 oncogene homolog (c-JUN) NH 2-terminal kinase protein (JNK), the activation of caspases, the increase in B cell leukemia/lymphoma 2 (Bcl-2) associated X protein (Bax), the decrease in Bcl xL protein (Bcl-XL) levels, the loss of mitochondria membrane potential and the release of cytochrome c. Conclusions: Abrogation of the G2 checkpoint should be an effective strategy against p53-deficient leukemia cells to irradiation-induced cell killing.

Original languageEnglish
Pages (from-to)97-109
Number of pages13
JournalInternational Journal of Radiation Biology
Volume82
Issue number2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • Apoptosis
  • Cell cycle checkpoints
  • Chemical modifiers
  • Leukaemias
  • Radiotherapy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

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