Abstract

Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p 

Original languageEnglish
Pages (from-to)302-308
JournalElectromagnetic Biology and Medicine
Volume34
Issue number4
DOIs
Publication statusPublished - 2015

Fingerprint

Dental Pulp
Cryopreservation
Magnetic Fields
Dimethyl Sulfoxide
Stem Cells
Freezing
Tooth
Cryoprotective Agents
Membrane Fluidity
Regenerative Medicine
Flow Cytometry

Keywords

  • Cryobiology
  • cryopreservation
  • dental pulp stem cell
  • static magnetic field

ASJC Scopus subject areas

  • Biophysics
  • Medicine (miscellaneous)

Cite this

Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation. / Lin, Shu Li; Chang, Wei-Jen; Lin, Chun Yen; Hsien, Sung-Chih; Lee, Sheng-Yang; Fan, Kang Hsin; Lin, Che-Tong; Huang, Haw-Ming.

In: Electromagnetic Biology and Medicine, Vol. 34, No. 4, 2015, p. 302-308.

Research output: Contribution to journalArticle

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