Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate

Sheng-Wei Feng, Yi June Lo, Wei Jen Chang, Che Tong Lin, Sheng Yang Lee, Yoshimitsu Abiko, Haw Ming Huang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-l-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P <0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype.

Original languageEnglish
Pages (from-to)793-798
Number of pages6
JournalMedical and Biological Engineering and Computing
Volume48
Issue number8
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Magnetic Fields
Cell Differentiation
Cells
Magnetic fields
Substrates
Phosphatases
Extracellular Matrix
Alkaline Phosphatase
poly(lactide)
Electron Scanning Microscopy
Cultured Cells
Assays
Phenotype
Scanning electron microscopy

Keywords

  • Differentiation
  • MG63
  • PLLA
  • Static magnetic field

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computer Science Applications
  • Medicine(all)

Cite this

@article{70ed0b4fd42f482b9a48bf0b7ae5645d,
title = "Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate",
abstract = "This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-l-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P <0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype.",
keywords = "Differentiation, MG63, PLLA, Static magnetic field",
author = "Sheng-Wei Feng and Lo, {Yi June} and Chang, {Wei Jen} and Lin, {Che Tong} and Lee, {Sheng Yang} and Yoshimitsu Abiko and Huang, {Haw Ming}",
year = "2010",
month = "8",
doi = "10.1007/s11517-010-0639-5",
language = "English",
volume = "48",
pages = "793--798",
journal = "Medical and Biological Engineering and Computing",
issn = "0140-0118",
publisher = "Peter Peregrinus Ltd",
number = "8",

}

TY - JOUR

T1 - Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate

AU - Feng, Sheng-Wei

AU - Lo, Yi June

AU - Chang, Wei Jen

AU - Lin, Che Tong

AU - Lee, Sheng Yang

AU - Abiko, Yoshimitsu

AU - Huang, Haw Ming

PY - 2010/8

Y1 - 2010/8

N2 - This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-l-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P <0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype.

AB - This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-l-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P <0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype.

KW - Differentiation

KW - MG63

KW - PLLA

KW - Static magnetic field

UR - http://www.scopus.com/inward/record.url?scp=77955466854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955466854&partnerID=8YFLogxK

U2 - 10.1007/s11517-010-0639-5

DO - 10.1007/s11517-010-0639-5

M3 - Article

VL - 48

SP - 793

EP - 798

JO - Medical and Biological Engineering and Computing

JF - Medical and Biological Engineering and Computing

SN - 0140-0118

IS - 8

ER -