Static magnetic field-enhanced osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells via matrix vesicle secretion

Ching Yi Chang, Wei Zhen Lew, Sheng Wei Feng, Chung Lung Wu, Hsin Hui Wang, Sung Chih Hsieh, Haw Ming Huang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

METHODS: In methodology, WJMSCs were treated with a 0.4-T SMF. The cell viability was tested using the MTT assay. For the osteogenic analysis, the alkaline phosphatase activity assay and alizarin red S staining were performed. The osteogenic-related gene expression of ALP, BMP-2, and Runx2 was examined using real-time polymerase chain reaction. Scanning electron microscopy combined with energy-dispersive X-ray spectroscopy was used to analyze matrix vesicle secretion.

RESULTS: The cell viability showed no significant difference between the SMF-treated group and the sham-exposed cells. However, the SMF-treated group exhibited significantly more mineralized nodule formation and higher ALP activity than their control counterparts ( p  < .05). The expressions of osteogenic-related markers, ALP, BMP-2, and Runx2, were also significantly higher in the SMF-treated WJMSCs. The scanning electron microscopy results showed much more matrix vesicle secretion in the SMF-treated cells than in the sham-treated cells. A mineralized sheath was noted in the SMF-treated cells, along with a sporadic accumulation of spherical mineralized deposits on the cell surface.

CONCLUSIONS: The results suggest that 0.4-T SMF treatment enhances the osteogenesis of WJMSCs at the early-to-middle stage of osteogenic differentiation by increasing the matrix vesicle secretion and mineralization.

Original languageEnglish
Pages (from-to)1207-1217
Number of pages11
JournalInternational Journal of Radiation Biology
Volume96
Issue number9
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Differentiation
  • matrix vesicle
  • static magnetic field
  • umbilical cord-derived mesenchymal stem cells
  • Wharton jelly

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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