Static magnetic field attenuates lipopolysaccharide-induced inflammation in pulp cells by affecting cell membrane stability

Sung Chih Hsieh, Jeng Ting Tsao, Wei Zhen Lew, Ya Hui Chan, Lin Wen Lee, Che Tong Lin, Yung Kai Huang, Haw Ming Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

One of the causes of dental pulpitis is lipopolysaccharide-(LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.

Original languageEnglish
Article number492683
JournalScientific World Journal
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Dental Pulp
Magnetic Fields
Cell membranes
Pulp
Lipopolysaccharides
Cell Membrane
Magnetic fields
membrane
magnetic field
Inflammation
induced response
Pulpitis
Tooth
Anisotropy
Stem cells
repair
Stem Cells
Assays
anisotropy
Repair

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Static magnetic field attenuates lipopolysaccharide-induced inflammation in pulp cells by affecting cell membrane stability. / Hsieh, Sung Chih; Tsao, Jeng Ting; Lew, Wei Zhen; Chan, Ya Hui; Lee, Lin Wen; Lin, Che Tong; Huang, Yung Kai; Huang, Haw Ming.

In: Scientific World Journal, Vol. 2015, 492683, 2015.

Research output: Contribution to journalArticle

Hsieh, Sung Chih ; Tsao, Jeng Ting ; Lew, Wei Zhen ; Chan, Ya Hui ; Lee, Lin Wen ; Lin, Che Tong ; Huang, Yung Kai ; Huang, Haw Ming. / Static magnetic field attenuates lipopolysaccharide-induced inflammation in pulp cells by affecting cell membrane stability. In: Scientific World Journal. 2015 ; Vol. 2015.
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