Abstract
Study objectives: Ischemia (I)/reperfusion (R) lung injury is an important clinical issue in lung transplantation. In the present study, we observed the effects of lung static inflation, different perfusates, and ventilatory gas with nitrogen or oxygen on the I/R-induced pulmonary damage. Design and setting: A total of 96 male Sprague-Dawley rats were used. The lung was isolated in situ. Methods: In an isolated lung, the capillary filtration coefficient (Kfc), lung weight gain (LWG), lung weight (LW)/body weight (BW) ratio, and protein concentration in BAL fluid (PCBAL) were measured or calculated to evaluate the degree of lung injury. Histologic examinations with hematoxylin-eosin staining were performed. Results: I/R caused lung injury, as reflected by increases in Kfc, LWG, LW/BW, and PCBAL. The histopathologic picture revealed the presence of hyaline membrane formation and the infiltration of inflammatory cells. These values were significantly attenuated by static lung inflation. The I/R lung damage appeared to be less in the lung perfused with whole blood than in the lung perfused with an isotonic solution. Therapy with ventilatory air (ie, nitrogen or oxygen) did not alter the I/R lung damage. Conclusions: The data suggest that lung inflation is protective to I/R injury, irrespective of the type of ventilatory air used for treatment. Tine preservation of the lung for transplantation is better kept at a static inflation state and perfused with whole blood instead of an isotonic physiologic solution.
| Original language | English |
|---|---|
| Pages (from-to) | 552-558 |
| Number of pages | 7 |
| Journal | Chest |
| Volume | 126 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2004 |
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Keywords
- Ischemia/reperfusion
- Lung injury
- Microvascular permeability
- Perfusate
- Static inflation
- Ventilatory air
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ. / Shang, Jyh Kao; Wang, David; Yeh, Diana Yu Wung; Hsu, Kang; Yung, Hsiang Hsu; Chen, Hsing I.
In: Chest, Vol. 126, No. 2, 08.2004, p. 552-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ
AU - Shang, Jyh Kao
AU - Wang, David
AU - Yeh, Diana Yu Wung
AU - Hsu, Kang
AU - Yung, Hsiang Hsu
AU - Chen, Hsing I.
PY - 2004/8
Y1 - 2004/8
N2 - Study objectives: Ischemia (I)/reperfusion (R) lung injury is an important clinical issue in lung transplantation. In the present study, we observed the effects of lung static inflation, different perfusates, and ventilatory gas with nitrogen or oxygen on the I/R-induced pulmonary damage. Design and setting: A total of 96 male Sprague-Dawley rats were used. The lung was isolated in situ. Methods: In an isolated lung, the capillary filtration coefficient (Kfc), lung weight gain (LWG), lung weight (LW)/body weight (BW) ratio, and protein concentration in BAL fluid (PCBAL) were measured or calculated to evaluate the degree of lung injury. Histologic examinations with hematoxylin-eosin staining were performed. Results: I/R caused lung injury, as reflected by increases in Kfc, LWG, LW/BW, and PCBAL. The histopathologic picture revealed the presence of hyaline membrane formation and the infiltration of inflammatory cells. These values were significantly attenuated by static lung inflation. The I/R lung damage appeared to be less in the lung perfused with whole blood than in the lung perfused with an isotonic solution. Therapy with ventilatory air (ie, nitrogen or oxygen) did not alter the I/R lung damage. Conclusions: The data suggest that lung inflation is protective to I/R injury, irrespective of the type of ventilatory air used for treatment. Tine preservation of the lung for transplantation is better kept at a static inflation state and perfused with whole blood instead of an isotonic physiologic solution.
AB - Study objectives: Ischemia (I)/reperfusion (R) lung injury is an important clinical issue in lung transplantation. In the present study, we observed the effects of lung static inflation, different perfusates, and ventilatory gas with nitrogen or oxygen on the I/R-induced pulmonary damage. Design and setting: A total of 96 male Sprague-Dawley rats were used. The lung was isolated in situ. Methods: In an isolated lung, the capillary filtration coefficient (Kfc), lung weight gain (LWG), lung weight (LW)/body weight (BW) ratio, and protein concentration in BAL fluid (PCBAL) were measured or calculated to evaluate the degree of lung injury. Histologic examinations with hematoxylin-eosin staining were performed. Results: I/R caused lung injury, as reflected by increases in Kfc, LWG, LW/BW, and PCBAL. The histopathologic picture revealed the presence of hyaline membrane formation and the infiltration of inflammatory cells. These values were significantly attenuated by static lung inflation. The I/R lung damage appeared to be less in the lung perfused with whole blood than in the lung perfused with an isotonic solution. Therapy with ventilatory air (ie, nitrogen or oxygen) did not alter the I/R lung damage. Conclusions: The data suggest that lung inflation is protective to I/R injury, irrespective of the type of ventilatory air used for treatment. Tine preservation of the lung for transplantation is better kept at a static inflation state and perfused with whole blood instead of an isotonic physiologic solution.
KW - Ischemia/reperfusion
KW - Lung injury
KW - Microvascular permeability
KW - Perfusate
KW - Static inflation
KW - Ventilatory air
UR - http://www.scopus.com/inward/record.url?scp=4143091518&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4143091518&partnerID=8YFLogxK
U2 - 10.1378/chest.126.2.552
DO - 10.1378/chest.126.2.552
M3 - Article
C2 - 15302744
AN - SCOPUS:4143091518
VL - 126
SP - 552
EP - 558
JO - Chest
JF - Chest
SN - 0012-3692
IS - 2
ER -