Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ

Jyh Kao Shang, David Wang, Diana Yu Wung Yeh, Kang Hsu, Hsiang Hsu Yung, Hsing I. Chen

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Study objectives: Ischemia (I)/reperfusion (R) lung injury is an important clinical issue in lung transplantation. In the present study, we observed the effects of lung static inflation, different perfusates, and ventilatory gas with nitrogen or oxygen on the I/R-induced pulmonary damage. Design and setting: A total of 96 male Sprague-Dawley rats were used. The lung was isolated in situ. Methods: In an isolated lung, the capillary filtration coefficient (Kfc), lung weight gain (LWG), lung weight (LW)/body weight (BW) ratio, and protein concentration in BAL fluid (PCBAL) were measured or calculated to evaluate the degree of lung injury. Histologic examinations with hematoxylin-eosin staining were performed. Results: I/R caused lung injury, as reflected by increases in Kfc, LWG, LW/BW, and PCBAL. The histopathologic picture revealed the presence of hyaline membrane formation and the infiltration of inflammatory cells. These values were significantly attenuated by static lung inflation. The I/R lung damage appeared to be less in the lung perfused with whole blood than in the lung perfused with an isotonic solution. Therapy with ventilatory air (ie, nitrogen or oxygen) did not alter the I/R lung damage. Conclusions: The data suggest that lung inflation is protective to I/R injury, irrespective of the type of ventilatory air used for treatment. Tine preservation of the lung for transplantation is better kept at a static inflation state and perfused with whole blood instead of an isotonic physiologic solution.

Original languageEnglish
Pages (from-to)552-558
Number of pages7
JournalChest
Volume126
Issue number2
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Economic Inflation
Reperfusion Injury
Lung
Lung Injury
Isotonic Solutions
Dimercaprol
Lung Transplantation
Weight Gain
Nitrogen
Air
Body Weight
Oxygen
Weights and Measures
Hyalin
Hematoxylin
Eosine Yellowish-(YS)
Reperfusion
Sprague Dawley Rats
Proteins
Ischemia

Keywords

  • Ischemia/reperfusion
  • Lung injury
  • Microvascular permeability
  • Perfusate
  • Static inflation
  • Ventilatory air

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Shang, J. K., Wang, D., Yeh, D. Y. W., Hsu, K., Yung, H. H., & Chen, H. I. (2004). Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ. Chest, 126(2), 552-558. https://doi.org/10.1378/chest.126.2.552

Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ. / Shang, Jyh Kao; Wang, David; Yeh, Diana Yu Wung; Hsu, Kang; Yung, Hsiang Hsu; Chen, Hsing I.

In: Chest, Vol. 126, No. 2, 08.2004, p. 552-558.

Research output: Contribution to journalArticle

Shang, JK, Wang, D, Yeh, DYW, Hsu, K, Yung, HH & Chen, HI 2004, 'Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ', Chest, vol. 126, no. 2, pp. 552-558. https://doi.org/10.1378/chest.126.2.552
Shang, Jyh Kao ; Wang, David ; Yeh, Diana Yu Wung ; Hsu, Kang ; Yung, Hsiang Hsu ; Chen, Hsing I. / Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ. In: Chest. 2004 ; Vol. 126, No. 2. pp. 552-558.
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