Stability, human blood distribution and rat tissue localization of promazine and desmonomethylpromazine

Oliver Y‐P Hu, Stephen H. Curry

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The stability in human blood and urine, partitioning into red blood cells and plasma protein binding of promazine and desmonomethylpromazine were investigated. Tissue localization was investigated in rats. Promazine and desmonomethylpromazine were stable in human plasma and urine for at least 64 days at −20°. The percentage of promazine not bound to protein in plasma was 10·4 ± 2·43 as estimated by equilibrium dialysis with correction for volume shift, and 11·6 ± 0·43 per cent as estimated by ultracentrifugation. Data for the mean plasma/red blood cell concentration ratio and the red blood cell/plasma distribution coefficient for promazine were 1·19 ± 0·13 and 8·21 ± 0·40, respectively. There was no evidence of time‐dependence in plasma/red blood cell partitioning. Ten rat organs and tissues were examined. The concentrations of promazine and desmonemethylpromazine were highest in lung. For promazine, the rank order of tissue localization was lung > liver > kidney > intestine > brain > spleen > red blood cell > voluntary muscle > plasma > stomach > heart. For desmonomethylpromazine, the order was reversed in the cases of spleen and brain and interchanged in the cases of stomach and muscle. The brain/plasma concentration ratios for promazine and desmonomethylpromazine in rat were 4·69 and 3·87, respectively.

Original languageEnglish
Pages (from-to)537-548
Number of pages12
JournalBiopharmaceutics & Drug Disposition
Volume10
Issue number6
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

Fingerprint

Promazine
Tissue Distribution
Erythrocytes
Blood Proteins
Stomach
Brain
Spleen
Urine
Lung
Ultracentrifugation
desmonomethylpromazine
Protein Binding
Intestines
Dialysis
Skeletal Muscle
Kidney
Muscles
Liver

Keywords

  • Desmonomethylpromazine
  • Plasma protein binding
  • Promazine
  • Red cell participation
  • Stability
  • Tissue localization

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Stability, human blood distribution and rat tissue localization of promazine and desmonomethylpromazine. / Hu, Oliver Y‐P; Curry, Stephen H.

In: Biopharmaceutics & Drug Disposition, Vol. 10, No. 6, 01.01.1989, p. 537-548.

Research output: Contribution to journalArticle

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