Src contributes to IL6-induced vascular endothelial growth factor-C expression in lymphatic endothelial cells

Yu Han Huang, Hung-Yu Yang, Ya Fen Hsu, Pei Ting Chiu, George Ou, Ming-Jen Hsu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Formation of lymphatic capillaries by lymphatic endothelial cells (LECs) occurs both in normal tissues as well as in pathological processes including tumor metastasis. Interleukin-6 (IL-6), a potent pro-inflammatory cytokine, has been shown to be highly elevated in various cancers. IL-6 has also been shown to increase tumor lymphangiogenesis through vascular endothelial growth factor-C (VEGF-C) induction in tumor cells. Although lymphangiogenesis is associated with lymph node metastasis and also resistance to conventional therapy in various cancers, the precise mechanisms of lymphangiogenesis in LECs remain unclear. This study aimed to investigate the signaling cascade involved in IL-6-induced VEGF-C expression in murine LECs (SV-LEC). The VEGF-C mRNA and protein levels were increased in SV-LECs exposed to IL-6. IL-6 time-dependently induced Src phosphorylation and downstream phosphorylation of ERK1/2 and p38MAPK. In contrast, PP2, an inhibitor of Src signaling, abrogated IL-6's effects on ERK1/2 and p38MAPK phosphorylation. IL-6 exposure also led to increase in VEGF-C promoter-luciferase activity as well as C/EBPβ- and κB-luciferase activities. VEGF-C promoter-, C/EBPβ- and κB-luciferase activities were all suppressed by Src, ERK1/2 or p38MAPK signaling blockades despite presence of IL-6. Finally, C/EBPβ and p65 binding to the VEGF-C promoter region were increased after IL-6 exposure in SV-LECs. Taken together, we report a Src-mediated ERK1/2 and p38MAPK activation resulting in C/EBPβ and p65 binding to the promoter region of VEGF-C, leading to VEGF-C expression in IL-6-exposed SV-LECs.

Original languageEnglish
Pages (from-to)407-418
Number of pages12
JournalAngiogenesis
Volume17
Issue number2
DOIs
Publication statusPublished - Apr 1 2014

Fingerprint

Vascular Endothelial Growth Factor C
Endothelial cells
Interleukin-6
Endothelial Cells
Lymphangiogenesis
Phosphorylation
Luciferases
Tumors
Neoplasms
Genetic Promoter Regions
Neoplasm Metastasis
Lymphatic Vessels
Pathologic Processes
Lymph Nodes
Chemical activation
Cells
Tissue
Cytokines

Keywords

  • Interleukin-6 (IL-6)
  • Lymphangiogenesis
  • Lymphatic endothelial cells (LECs)
  • Src
  • Vascular endothelial growth factor-C (VEGF-C)

ASJC Scopus subject areas

  • Cancer Research
  • Physiology
  • Clinical Biochemistry
  • Medicine(all)

Cite this

Src contributes to IL6-induced vascular endothelial growth factor-C expression in lymphatic endothelial cells. / Huang, Yu Han; Yang, Hung-Yu; Hsu, Ya Fen; Chiu, Pei Ting; Ou, George; Hsu, Ming-Jen.

In: Angiogenesis, Vol. 17, No. 2, 01.04.2014, p. 407-418.

Research output: Contribution to journalArticle

Huang, Yu Han ; Yang, Hung-Yu ; Hsu, Ya Fen ; Chiu, Pei Ting ; Ou, George ; Hsu, Ming-Jen. / Src contributes to IL6-induced vascular endothelial growth factor-C expression in lymphatic endothelial cells. In: Angiogenesis. 2014 ; Vol. 17, No. 2. pp. 407-418.
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abstract = "Formation of lymphatic capillaries by lymphatic endothelial cells (LECs) occurs both in normal tissues as well as in pathological processes including tumor metastasis. Interleukin-6 (IL-6), a potent pro-inflammatory cytokine, has been shown to be highly elevated in various cancers. IL-6 has also been shown to increase tumor lymphangiogenesis through vascular endothelial growth factor-C (VEGF-C) induction in tumor cells. Although lymphangiogenesis is associated with lymph node metastasis and also resistance to conventional therapy in various cancers, the precise mechanisms of lymphangiogenesis in LECs remain unclear. This study aimed to investigate the signaling cascade involved in IL-6-induced VEGF-C expression in murine LECs (SV-LEC). The VEGF-C mRNA and protein levels were increased in SV-LECs exposed to IL-6. IL-6 time-dependently induced Src phosphorylation and downstream phosphorylation of ERK1/2 and p38MAPK. In contrast, PP2, an inhibitor of Src signaling, abrogated IL-6's effects on ERK1/2 and p38MAPK phosphorylation. IL-6 exposure also led to increase in VEGF-C promoter-luciferase activity as well as C/EBPβ- and κB-luciferase activities. VEGF-C promoter-, C/EBPβ- and κB-luciferase activities were all suppressed by Src, ERK1/2 or p38MAPK signaling blockades despite presence of IL-6. Finally, C/EBPβ and p65 binding to the VEGF-C promoter region were increased after IL-6 exposure in SV-LECs. Taken together, we report a Src-mediated ERK1/2 and p38MAPK activation resulting in C/EBPβ and p65 binding to the promoter region of VEGF-C, leading to VEGF-C expression in IL-6-exposed SV-LECs.",
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AU - Huang, Yu Han

AU - Yang, Hung-Yu

AU - Hsu, Ya Fen

AU - Chiu, Pei Ting

AU - Ou, George

AU - Hsu, Ming-Jen

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AB - Formation of lymphatic capillaries by lymphatic endothelial cells (LECs) occurs both in normal tissues as well as in pathological processes including tumor metastasis. Interleukin-6 (IL-6), a potent pro-inflammatory cytokine, has been shown to be highly elevated in various cancers. IL-6 has also been shown to increase tumor lymphangiogenesis through vascular endothelial growth factor-C (VEGF-C) induction in tumor cells. Although lymphangiogenesis is associated with lymph node metastasis and also resistance to conventional therapy in various cancers, the precise mechanisms of lymphangiogenesis in LECs remain unclear. This study aimed to investigate the signaling cascade involved in IL-6-induced VEGF-C expression in murine LECs (SV-LEC). The VEGF-C mRNA and protein levels were increased in SV-LECs exposed to IL-6. IL-6 time-dependently induced Src phosphorylation and downstream phosphorylation of ERK1/2 and p38MAPK. In contrast, PP2, an inhibitor of Src signaling, abrogated IL-6's effects on ERK1/2 and p38MAPK phosphorylation. IL-6 exposure also led to increase in VEGF-C promoter-luciferase activity as well as C/EBPβ- and κB-luciferase activities. VEGF-C promoter-, C/EBPβ- and κB-luciferase activities were all suppressed by Src, ERK1/2 or p38MAPK signaling blockades despite presence of IL-6. Finally, C/EBPβ and p65 binding to the VEGF-C promoter region were increased after IL-6 exposure in SV-LECs. Taken together, we report a Src-mediated ERK1/2 and p38MAPK activation resulting in C/EBPβ and p65 binding to the promoter region of VEGF-C, leading to VEGF-C expression in IL-6-exposed SV-LECs.

KW - Interleukin-6 (IL-6)

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KW - Src

KW - Vascular endothelial growth factor-C (VEGF-C)

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