Spray-dried microparticles containing polymeric micelles encapsulating hematoporphyrin

Yu Tsai Yang, Chin Tin Chen, Jen Chang Yang, Tsuimin Tsai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of this study was to examine the properties of a new pulmonary delivery platform of microparticles containing micelles in which a therapeutic photosensitizing drug, hematoporphyrin (Hp), was encapsulated. Different poloxamers were used to form micellar Hp, and one of these, Pluronic L122-Hp, was subsequently incorporated into lactose microparticles by spray-drying. Spectral and morphological analyses were performed on both micellar Hp, and lactose microparticles containing micellar Hp (lactose-micellar Hp) before and after dissolution of the microparticles in water. Photodynamic activity of the various Hp samples were evaluated in human lung epithelial carcinoma A549 cells using a light-emitting diode (LED) device at a wavelength of 630±5 nm. No significant difference was observed between micellar Hp and lactose-micellar Hp regarding the generation of singlet oxygen. The mean particle size of the microparticles was 2.3±0.7μm which is within the size range for potential lung delivery. The cellular uptake of micellar Hp and lactose-micellar Hp measured on A549 cells was at least twofold higher than those obtained with the Hp at equivalent concentrations. Micellar Hp exhibited higher cytotoxicity than Hp due to reduced formation of Hp aggregates and increased cellar uptake. The spectral properties as well as the photodynamic activity of the micellar Hp was retained when formulated into microparticles by spray-drying. Microparticles containing micelles have the potential for delivering micelle-encapsulated hydrophobic drugs in targeted therapy of pulmonary diseases.

Original languageEnglish
Pages (from-to)138-146
Number of pages9
JournalAAPS Journal
Volume12
Issue number2
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Hematoporphyrins
Micelles
Lactose
Poloxamer
Lung
Singlet Oxygen
Particle Size

Keywords

  • Micelle
  • Microparticle
  • Photosensitizer
  • Pulmonary
  • Spray-drying

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Spray-dried microparticles containing polymeric micelles encapsulating hematoporphyrin. / Yang, Yu Tsai; Chen, Chin Tin; Yang, Jen Chang; Tsai, Tsuimin.

In: AAPS Journal, Vol. 12, No. 2, 06.2010, p. 138-146.

Research output: Contribution to journalArticle

Yang, Yu Tsai ; Chen, Chin Tin ; Yang, Jen Chang ; Tsai, Tsuimin. / Spray-dried microparticles containing polymeric micelles encapsulating hematoporphyrin. In: AAPS Journal. 2010 ; Vol. 12, No. 2. pp. 138-146.
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