Abstract
The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Renal Physiology |
| Volume | 293 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2007 |
| Externally published | Yes |
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Keywords
- Glutamate
- Posttetanic potentiation
- Postvoiding potentiation
ASJC Scopus subject areas
- Physiology
Cite this
Spinal glutamatergic NMDA-dependent cyclic pelvic nerve-to-external urethra sphincter reflex potentiation in anesthetized rats. / Liao, Jiuan Miaw; Yang, Cho Hsun; Cheng, Chen Li; Pan, Shwu Fen; Chen, Mei Jung; Huang, Pei Chen; Chen, Gin Den; Tung, Kwong Chung; Peng, Hsien Yu; Lin, Tzer Bin.
In: American Journal of Physiology - Renal Physiology, Vol. 293, No. 3, 09.2007.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spinal glutamatergic NMDA-dependent cyclic pelvic nerve-to-external urethra sphincter reflex potentiation in anesthetized rats
AU - Liao, Jiuan Miaw
AU - Yang, Cho Hsun
AU - Cheng, Chen Li
AU - Pan, Shwu Fen
AU - Chen, Mei Jung
AU - Huang, Pei Chen
AU - Chen, Gin Den
AU - Tung, Kwong Chung
AU - Peng, Hsien Yu
AU - Lin, Tzer Bin
PY - 2007/9
Y1 - 2007/9
N2 - The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.
AB - The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.
KW - Glutamate
KW - Posttetanic potentiation
KW - Postvoiding potentiation
UR - http://www.scopus.com/inward/record.url?scp=34548618748&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548618748&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.00296.2006
DO - 10.1152/ajprenal.00296.2006
M3 - Article
C2 - 17376759
AN - SCOPUS:34548618748
VL - 293
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
SN - 1931-857X
IS - 3
ER -