Spinal glutamatergic NMDA-dependent cyclic pelvic nerve-to-external urethra sphincter reflex potentiation in anesthetized rats

Jiuan Miaw Liao, Cho Hsun Yang, Chen Li Cheng, Shwu Fen Pan, Mei Jung Chen, Pei Chen Huang, Gin Den Chen, Kwong Chung Tung, Hsien Yu Peng, Tzer Bin Lin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume293
Issue number3
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

Fingerprint

Urethra
N-Methylaspartate
Reflex
6-Cyano-7-nitroquinoxaline-2,3-dione
Pressure
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Synaptic Transmission
Urinary Bladder

Keywords

  • Glutamate
  • Posttetanic potentiation
  • Postvoiding potentiation

ASJC Scopus subject areas

  • Physiology

Cite this

Spinal glutamatergic NMDA-dependent cyclic pelvic nerve-to-external urethra sphincter reflex potentiation in anesthetized rats. / Liao, Jiuan Miaw; Yang, Cho Hsun; Cheng, Chen Li; Pan, Shwu Fen; Chen, Mei Jung; Huang, Pei Chen; Chen, Gin Den; Tung, Kwong Chung; Peng, Hsien Yu; Lin, Tzer Bin.

In: American Journal of Physiology - Renal Physiology, Vol. 293, No. 3, 09.2007.

Research output: Contribution to journalArticle

Liao, Jiuan Miaw ; Yang, Cho Hsun ; Cheng, Chen Li ; Pan, Shwu Fen ; Chen, Mei Jung ; Huang, Pei Chen ; Chen, Gin Den ; Tung, Kwong Chung ; Peng, Hsien Yu ; Lin, Tzer Bin. / Spinal glutamatergic NMDA-dependent cyclic pelvic nerve-to-external urethra sphincter reflex potentiation in anesthetized rats. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 293, No. 3.
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abstract = "The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.",
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T1 - Spinal glutamatergic NMDA-dependent cyclic pelvic nerve-to-external urethra sphincter reflex potentiation in anesthetized rats

AU - Liao, Jiuan Miaw

AU - Yang, Cho Hsun

AU - Cheng, Chen Li

AU - Pan, Shwu Fen

AU - Chen, Mei Jung

AU - Huang, Pei Chen

AU - Chen, Gin Den

AU - Tung, Kwong Chung

AU - Peng, Hsien Yu

AU - Lin, Tzer Bin

PY - 2007/9

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N2 - The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.

AB - The purposes of this study were to investigate whether the pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced under physiological conditions and to determine whether glutamatergic neurotransmission is involved in the reflex potentiation. Stimulation-evoked reflex activities, during rhythmic bladder contractions caused by a continuous saline infusion, in 21 anesthetized rats were recorded with/without the intrathecal administration of 10 μl of CNQX (a glutamatergic AMPA receptor antagonist; 100 μM) and APV( a glutamatergic NMDA receptor antagonist; 100 μM). Reflex activities became potentiated following the increment of intravesical pressure (IVP) during the storage phase (2.39 ± 0.28 spikes/mmHg, n = 21) and the ascending period of the voiding phase (1.46 ± 0.35 spikes/ mmHg, n = 21) and decreased following the decrement of IVP during the descending period of the voiding phase (1.50 ± 0.33 spikes/ mmHg, n = 21). Although it is characterized by a low IVP, a postvoiding reflex potentiation in stimulation-evoked activities was elicited at the critical period after a voiding contraction had just finished (23.95 ± 8.96 spikes/mmHg, n = 21). The slope of the regression line of evoked activities vs. the IVP during the storage phase was significantly (P <0.01) higher than that of the ascending and descending periods of the voiding phase, but there was no statistical difference between the ascending and the descending periods (P > 0.05). In addition, the slope of the regression line of posttetanic reflex potentiation was significantly higher than that of the storage phase (P <0.01). All the slopes of the regression lines decreased after intrathecal CNQX administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.83 ± 0.31, 0.74 ± 0.12, 0.76 ± 0.12, and 4.31 ± 3.71 spikes/mmHg in storage, ascending and descending period of the voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). The slopes of the regression lines became almost horizontal after intrathecal APV administration (from 3.15 ± 0.44, 2.10 ± 0.57, 2.13 ± 0.53, and 21.30 ± 3.41 to 0.16 ± 0.12, 0.21 ± 0.07, 0.18 ± 0.05, and 0.23 ± 0.76 spikes/mmHg in storage, ascending and descending period of voiding phase, and postvoiding potentiation, respectively; all P <0.01, n = 10). Our results suggest that a potentiation in the pelvic nerve-to-EUS reflex can be induced under physiological conditions and the glutamatergic mechanism appears to be involved in this reflex potentiation.

KW - Glutamate

KW - Posttetanic potentiation

KW - Postvoiding potentiation

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