Sphere-Formation Assay: Three-dimensional in vitro culturing of prostate cancer stem/Progenitor sphere-forming cells

Hisham F. Bahmad, Katia Cheaito, Reda M. Chalhoub, Ola Hadadeh, Alissar Monzer, Farah Ballout, Albert El-Hajj, Deborah Mukherji, Yen Nien Liu, Georges Daoud, Wassim Abou-Kheir

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cancer Stem Cells (CSCs) are a sub-population of cells, identified in most tumors, responsible for the initiation, recurrence, metastatic potential, and resistance of different malignancies. In prostate cancer (PCa), CSCs were identified and thought to be responsible for the generation of the lethal subtype, commonly known as Castration-Resistant Prostate Cancer (CRPC). In vitro models to investigate the properties of CSCs in PCa are highly required. Sphere-formation assay is an in vitro method commonly used to identify CSCs and study their properties. Here, we report the detailed methodology on how to generate and propagate spheres from PCa cell lines and from murine prostate tissue. This model is based on the ability of stem cells to grow in non-adherent serum-free gel matrix. We also describe how to use these spheres in histological and immuno-fluorescent staining assays to assess the differentiation potential of the CSCs. Our results show the sphere-formation Assay (SFA) as a reliable in vitro assay to assess the presence and self-renewal ability of CSCs in different PCa models. This platform presents a useful tool to evaluate the effect of conventional or novel agents on the initiation and self-renewing properties of different tumors. The effects can be directly evaluated through assessment of the sphere-forming efficiency (SFE) over five generations or other downstream assays such as immuno-histochemical analysis of the generated spheres.

Original languageEnglish
Article number347
JournalFrontiers in Oncology
Volume8
Issue numberAUG
DOIs
Publication statusPublished - Aug 28 2018

Fingerprint

Neoplastic Stem Cells
Prostatic Neoplasms
Neoplasms
Castration
In Vitro Techniques
Prostate
Stem Cells
Gels
Staining and Labeling
Recurrence
Cell Line
Serum
Population

Keywords

  • Cancer stem cells
  • Differentiation
  • Prostate cancer
  • Prostatospheres
  • Self-renewal
  • Sphere-formation assay

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Bahmad, H. F., Cheaito, K., Chalhoub, R. M., Hadadeh, O., Monzer, A., Ballout, F., ... Abou-Kheir, W. (2018). Sphere-Formation Assay: Three-dimensional in vitro culturing of prostate cancer stem/Progenitor sphere-forming cells. Frontiers in Oncology, 8(AUG), [347]. https://doi.org/10.3389/fonc.2018.00347

Sphere-Formation Assay : Three-dimensional in vitro culturing of prostate cancer stem/Progenitor sphere-forming cells. / Bahmad, Hisham F.; Cheaito, Katia; Chalhoub, Reda M.; Hadadeh, Ola; Monzer, Alissar; Ballout, Farah; El-Hajj, Albert; Mukherji, Deborah; Liu, Yen Nien; Daoud, Georges; Abou-Kheir, Wassim.

In: Frontiers in Oncology, Vol. 8, No. AUG, 347, 28.08.2018.

Research output: Contribution to journalArticle

Bahmad, HF, Cheaito, K, Chalhoub, RM, Hadadeh, O, Monzer, A, Ballout, F, El-Hajj, A, Mukherji, D, Liu, YN, Daoud, G & Abou-Kheir, W 2018, 'Sphere-Formation Assay: Three-dimensional in vitro culturing of prostate cancer stem/Progenitor sphere-forming cells', Frontiers in Oncology, vol. 8, no. AUG, 347. https://doi.org/10.3389/fonc.2018.00347
Bahmad, Hisham F. ; Cheaito, Katia ; Chalhoub, Reda M. ; Hadadeh, Ola ; Monzer, Alissar ; Ballout, Farah ; El-Hajj, Albert ; Mukherji, Deborah ; Liu, Yen Nien ; Daoud, Georges ; Abou-Kheir, Wassim. / Sphere-Formation Assay : Three-dimensional in vitro culturing of prostate cancer stem/Progenitor sphere-forming cells. In: Frontiers in Oncology. 2018 ; Vol. 8, No. AUG.
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