Spermatozoa recovered by IxaPrep gradient have improved longevity and better motion characteristics than those by percoll gradient

J. H. Yang, M. Y. Wu, C. D. Chen, K. H. Chao, S. U. Chen, H. N. Ho, Y. S. Yang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To compare the effectiveness of various sperm preparation methods, we examined sperm concentration, percentage of progressive motility, recovery rate, and various motion characteristics in 32 semen samples after Percoll and IxaPrep preparations. Except for sperm concentration, which was much higher after IxaPrep preparation, no statistical differences existed between these two methods in terms of motile sperm concentration (MSC), recovery rate, mean curvilinear velocity (VCL), mean straight-line velocity (VSL) and mean amplitude of lateral head displacement (ALH). Among 22 samples that were allocated in a 37°C, 5% CO 2 incubator, the mean MSC after 3 hours was significantly higher following IxaPrep preparation than following Percoll preparation (63.2 x 10 6 /mL vs. 42.8 x 10 6 /mL, p < .03). VCL and VSL were much higher after IxaPrep preparation than after Percoll preparation, both at 3 hours (p < .01) and at 24 hours (p < .03). ALH was also higher after IxaPrep preparation than after Percoll preparation, and it was statistically significant after 3 hours of incubation (p < .03). We conclude that the IxaPrep gradient may be adopted as the preferred method to the Percoll gradient in preparing spermatozoa for assisted reproduction because of the spermatozoa's persistent and better motion activities after incubation.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalArchives of Andrology
Volume40
Issue number3
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

Fingerprint

Spermatozoa
Incubators
Carbon Monoxide
Percoll
Semen
Reproduction

Keywords

  • CASA
  • IxaPrep gradient
  • Percoll gradient

ASJC Scopus subject areas

  • Endocrinology

Cite this

Spermatozoa recovered by IxaPrep gradient have improved longevity and better motion characteristics than those by percoll gradient. / Yang, J. H.; Wu, M. Y.; Chen, C. D.; Chao, K. H.; Chen, S. U.; Ho, H. N.; Yang, Y. S.

In: Archives of Andrology, Vol. 40, No. 3, 01.01.1998, p. 237-245.

Research output: Contribution to journalArticle

Yang, J. H. ; Wu, M. Y. ; Chen, C. D. ; Chao, K. H. ; Chen, S. U. ; Ho, H. N. ; Yang, Y. S. / Spermatozoa recovered by IxaPrep gradient have improved longevity and better motion characteristics than those by percoll gradient. In: Archives of Andrology. 1998 ; Vol. 40, No. 3. pp. 237-245.
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