Sperm mitochondrial DNA depletion in men with asthenospermia

Shu Huei Kao, Hsiang Tai Chao, Hwan Wun Liu, Tien Lin Liao, Yau Huei Wei

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Objective To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Design Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. Setting University hospital infertility center. Patient(s) Eighty-six men who sought infertility therapy. Intervention(s) Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Main outcome measure(s) Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. Result(s) A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (

Original languageEnglish
Pages (from-to)66-73
Number of pages8
JournalFertility and Sterility
Volume82
Issue number1
DOIs
Publication statusPublished - Jul 2004

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Keywords

  • Depletion
  • infertility
  • mitochondrial DNA
  • motility
  • sperm

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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