Specific plant terpenoids and lignoids possess potent antiviral activities against severe acute respiratory syndrome coronavirus

Chih Chun Wen, Yueh Hsiung Kuo, Jia Tsrong Jan, Po Huang Liang, Sheng Yang Wang, Hong Gi Liu, Ching Kuo Lee, Shang Tzen Chang, Chih Jung Kuo, Shoei Sheng Lee, Chia Chung Hou, Pei Wen Hsiao, Shih Chang Chien, Lie Fen Shyur, Ning Sun Yang

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

In this study, 221 phytocompounds were evaluated for activity against anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) activities using a cell-based assay measuring SARS-CoV-induced cytopathogenic effect on Vero E6 cells. Ten diterpenoids (1-10), two sesquiterpenoids (11 and 12), two triterpenoids (13 and 14), five lignoids (15-19), curcumin (20), and reference controls niclosamide (21) and valinomycin (22) were potent inhibitors at concentrations between 3.3 and 10 μM. The concentrations of the 22 compounds to inhibit 50% of Vero E6 cell proliferation (CC50) and viral replication (EC50) were measured. The selective index values (SI = CC50/EC50) of the most potent compounds 1, 5, 6, 8, 14, and 16 were 58, >510, 111, 193, 180, and >667, respectively. Betulinic acid (13) and savinin (16) were competitive inhibitors of SARS-CoV 3CL protease with Ki values = 8.2 ± 0.7 and 9.1 ± 2.4 μM, respectively. Our findings suggest that specific abietane-type diterpenoids and lignoids exhibit strong anti-SARS-CoV effects.

Original languageEnglish
Pages (from-to)4087-4095
Number of pages9
JournalJournal of Medicinal Chemistry
Volume50
Issue number17
DOIs
Publication statusPublished - Aug 23 2007

ASJC Scopus subject areas

  • Organic Chemistry

Fingerprint Dive into the research topics of 'Specific plant terpenoids and lignoids possess potent antiviral activities against severe acute respiratory syndrome coronavirus'. Together they form a unique fingerprint.

  • Cite this

    Wen, C. C., Kuo, Y. H., Jan, J. T., Liang, P. H., Wang, S. Y., Liu, H. G., Lee, C. K., Chang, S. T., Kuo, C. J., Lee, S. S., Hou, C. C., Hsiao, P. W., Chien, S. C., Shyur, L. F., & Yang, N. S. (2007). Specific plant terpenoids and lignoids possess potent antiviral activities against severe acute respiratory syndrome coronavirus. Journal of Medicinal Chemistry, 50(17), 4087-4095. https://doi.org/10.1021/jm070295s