Specific cleavages of DNA by ascorbate in the presence of copper ion or copper chelates

Shyh Horng Chiou, Wen Chang Chang, Yuh Shan Jou, Hui Min M. Chung, Tung Bin Lo

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The DNA-cleavage specificity of ascorbate in the presence of copper ion is analyzed with end-labeled pBR322 DNA fragments. The nonenzymatic reaction of Cu(II)/ascorbate and DNA shows certain degrees of cleavage preference toward purinecontaining short segments in the labeled DNA under mild conditions (at 0°C and 10 min). The segments of pyrimidine clusters are least susceptible to cleavage. The DNA scission cannot be detected in the absence of metal ions, and is greatly diminished in the presence of EDTA and metal-chelating peptide. It is more specific than the nuclease-like scission activity induced by cuprous-phenanthroline complex. This scission activity in relation to the antiviral and antitumor activities of vitamin C reported in the literature deserves a crucial consideration.

Original languageEnglish
Pages (from-to)1723-1726
Number of pages4
JournalJournal of Biochemistry
Volume98
Issue number6
DOIs
Publication statusPublished - Jan 1 1985
Externally publishedYes

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DNA Cleavage
Copper
Ions
DNA
Metals
Phenanthrolines
Edetic Acid
Ascorbic Acid
Antiviral Agents
Chelation
Metal ions
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Specific cleavages of DNA by ascorbate in the presence of copper ion or copper chelates. / Chiou, Shyh Horng; Chang, Wen Chang; Jou, Yuh Shan; Chung, Hui Min M.; Lo, Tung Bin.

In: Journal of Biochemistry, Vol. 98, No. 6, 01.01.1985, p. 1723-1726.

Research output: Contribution to journalArticle

Chiou, Shyh Horng ; Chang, Wen Chang ; Jou, Yuh Shan ; Chung, Hui Min M. ; Lo, Tung Bin. / Specific cleavages of DNA by ascorbate in the presence of copper ion or copper chelates. In: Journal of Biochemistry. 1985 ; Vol. 98, No. 6. pp. 1723-1726.
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