Spatiotemporal changes of neuronal responses in the primary somatosensory cortex to noxious tail stimulation in awake and pentobarbital-anesthetized rats

Chung Chih Kuo, Jye Chang Lee, Ruei Jen Chiou, Meng Li Tsai, Chen Tung Yen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Primary somatosensory cortex (SI) is a key area in the processing of nociceptor inputs to our consciousness. To clarify the columnar and laminar organization of SI for pain processing, we compared spatiotemporal changes in neuronal activities of the primary sensorimotor cortex (SmI) of the rat in response to noxious laser heat stimulation applied to the mid-tail. Longitudinal and vertical array microelectrodes were chronically implanted in the cerebral cortex. Evoked neuronal activities, including intracortical local field potentials (LFP) and ensemble single-unit activity (SU) around SmI were simultaneously recorded. The effect of pentobarbital on the neuronal responses was evaluated in comparison with the neuronal responses in conscious animals to explore the potential substrate of nociceptive processing in the conscious state. The results from the experiment with longitudinal microelectrode arrays indicated that noxious stimulation induced a neuronal response which was spread widely around the SmI of the conscious rat, and the range of neuronal responses was limited to the tail region of the SmI under anesthesia. The results from the experiment with vertical microelectrode arrays showed the universal neuronal responses through all cortical layers of the SmI in conscious rats, and sodium pentobarbital suppressed these neuronal responses in the supragranular layers significantly relative to the deeper layers and basal activity. These results imply that a wider range of cortical activation, both in the horizontal or vertical dimension, might be important for nociceptive processing in the conscious state.

Original languageEnglish
Pages (from-to)332-342
Number of pages11
JournalChinese Journal of Physiology
Volume58
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Somatosensory Cortex
Microelectrodes
Pentobarbital
Tail
Vertical Dimension
Nociceptors
Consciousness
Cerebral Cortex
Lasers
Anesthesia
Hot Temperature
Pain

Keywords

  • Ensemble single-unit activity
  • Laser evoked potential
  • Microelectrode array
  • Nociception
  • Pain
  • Sodium pentobarbital

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Spatiotemporal changes of neuronal responses in the primary somatosensory cortex to noxious tail stimulation in awake and pentobarbital-anesthetized rats. / Kuo, Chung Chih; Lee, Jye Chang; Chiou, Ruei Jen; Tsai, Meng Li; Yen, Chen Tung.

In: Chinese Journal of Physiology, Vol. 58, No. 5, 2015, p. 332-342.

Research output: Contribution to journalArticle

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