Spatial heterogeneity of protein expression induced by dyssynchronous right ventricular pacing in the left ventricle of dogs with preserved systolic function

Jih Min Lin, Ling Ping Lai, Nai Kuan Chou, Jiunn Lee Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Right ventricular (RV) apical pacing may result in ventricular dyssynchrony, which is associated with functional and morphological changes in the left ventricle (LV). Our aim is to assess contraction and hypertrophy-related protein expression changes in the LV after RV apical pacing. Methods and Results: Six dogs underwent dual chamber pacemaker (DDD) implantation and atrioventricular nodal catheter ablation. The pacing group received atria-sensed RV apical pacing for 12 weeks. LV dyssynchrony was assessed with speckle tracking technique. Subsequently, hearts were processed for Western blotting. Four sham-operated dogs were included for comparison. After 12 weeks of RV pacing, cardiac chamber size and LV ejection fraction remained unchanged. Both electrical and mechanical dyssynchrony were evident in RV-paced dogs compared with sham-operated dogs. The late-activated LV lateral wall of paced dogs displayed a 23% reduction in the amount of sarcoplasmic reticulum Ca2+ ATPase, a 32% reduction in phospholamban levels, but a 3.6-fold increase in phospho-JNK expression, a 2.2-fold increase in phospho-p38, and 1.9-fold increase in phospho-ERK expression. There were no significant differences in the early-activated LV septum between paced and sham dogs. Conclusions: Temporal dispersion of mechanical activation by RV apical pacing induced spatial dispersion of protein expression in the LV.

Original languageEnglish
Pages (from-to)700-706
Number of pages7
JournalJournal of Cardiac Failure
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 1 2010
Externally publishedYes

Fingerprint

Heart Ventricles
Dogs
Proteins
Dichlorodiphenyldichloroethane
Catheter Ablation
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Heart Atria
Hypertrophy
Western Blotting

Keywords

  • calcium handling protein
  • Dyssynchrony
  • heart failure
  • mitogen-activated protein kinase
  • ventricular pacing

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Spatial heterogeneity of protein expression induced by dyssynchronous right ventricular pacing in the left ventricle of dogs with preserved systolic function. / Lin, Jih Min; Lai, Ling Ping; Chou, Nai Kuan; Lin, Jiunn Lee.

In: Journal of Cardiac Failure, Vol. 16, No. 8, 01.08.2010, p. 700-706.

Research output: Contribution to journalArticle

@article{647707c95bd347288d18e8ff6f08fcb6,
title = "Spatial heterogeneity of protein expression induced by dyssynchronous right ventricular pacing in the left ventricle of dogs with preserved systolic function",
abstract = "Background: Right ventricular (RV) apical pacing may result in ventricular dyssynchrony, which is associated with functional and morphological changes in the left ventricle (LV). Our aim is to assess contraction and hypertrophy-related protein expression changes in the LV after RV apical pacing. Methods and Results: Six dogs underwent dual chamber pacemaker (DDD) implantation and atrioventricular nodal catheter ablation. The pacing group received atria-sensed RV apical pacing for 12 weeks. LV dyssynchrony was assessed with speckle tracking technique. Subsequently, hearts were processed for Western blotting. Four sham-operated dogs were included for comparison. After 12 weeks of RV pacing, cardiac chamber size and LV ejection fraction remained unchanged. Both electrical and mechanical dyssynchrony were evident in RV-paced dogs compared with sham-operated dogs. The late-activated LV lateral wall of paced dogs displayed a 23{\%} reduction in the amount of sarcoplasmic reticulum Ca2+ ATPase, a 32{\%} reduction in phospholamban levels, but a 3.6-fold increase in phospho-JNK expression, a 2.2-fold increase in phospho-p38, and 1.9-fold increase in phospho-ERK expression. There were no significant differences in the early-activated LV septum between paced and sham dogs. Conclusions: Temporal dispersion of mechanical activation by RV apical pacing induced spatial dispersion of protein expression in the LV.",
keywords = "calcium handling protein, Dyssynchrony, heart failure, mitogen-activated protein kinase, ventricular pacing",
author = "Lin, {Jih Min} and Lai, {Ling Ping} and Chou, {Nai Kuan} and Lin, {Jiunn Lee}",
year = "2010",
month = "8",
day = "1",
doi = "10.1016/j.cardfail.2010.04.001",
language = "English",
volume = "16",
pages = "700--706",
journal = "Journal of Cardiac Failure",
issn = "1071-9164",
publisher = "Elsevier",
number = "8",

}

TY - JOUR

T1 - Spatial heterogeneity of protein expression induced by dyssynchronous right ventricular pacing in the left ventricle of dogs with preserved systolic function

AU - Lin, Jih Min

AU - Lai, Ling Ping

AU - Chou, Nai Kuan

AU - Lin, Jiunn Lee

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Background: Right ventricular (RV) apical pacing may result in ventricular dyssynchrony, which is associated with functional and morphological changes in the left ventricle (LV). Our aim is to assess contraction and hypertrophy-related protein expression changes in the LV after RV apical pacing. Methods and Results: Six dogs underwent dual chamber pacemaker (DDD) implantation and atrioventricular nodal catheter ablation. The pacing group received atria-sensed RV apical pacing for 12 weeks. LV dyssynchrony was assessed with speckle tracking technique. Subsequently, hearts were processed for Western blotting. Four sham-operated dogs were included for comparison. After 12 weeks of RV pacing, cardiac chamber size and LV ejection fraction remained unchanged. Both electrical and mechanical dyssynchrony were evident in RV-paced dogs compared with sham-operated dogs. The late-activated LV lateral wall of paced dogs displayed a 23% reduction in the amount of sarcoplasmic reticulum Ca2+ ATPase, a 32% reduction in phospholamban levels, but a 3.6-fold increase in phospho-JNK expression, a 2.2-fold increase in phospho-p38, and 1.9-fold increase in phospho-ERK expression. There were no significant differences in the early-activated LV septum between paced and sham dogs. Conclusions: Temporal dispersion of mechanical activation by RV apical pacing induced spatial dispersion of protein expression in the LV.

AB - Background: Right ventricular (RV) apical pacing may result in ventricular dyssynchrony, which is associated with functional and morphological changes in the left ventricle (LV). Our aim is to assess contraction and hypertrophy-related protein expression changes in the LV after RV apical pacing. Methods and Results: Six dogs underwent dual chamber pacemaker (DDD) implantation and atrioventricular nodal catheter ablation. The pacing group received atria-sensed RV apical pacing for 12 weeks. LV dyssynchrony was assessed with speckle tracking technique. Subsequently, hearts were processed for Western blotting. Four sham-operated dogs were included for comparison. After 12 weeks of RV pacing, cardiac chamber size and LV ejection fraction remained unchanged. Both electrical and mechanical dyssynchrony were evident in RV-paced dogs compared with sham-operated dogs. The late-activated LV lateral wall of paced dogs displayed a 23% reduction in the amount of sarcoplasmic reticulum Ca2+ ATPase, a 32% reduction in phospholamban levels, but a 3.6-fold increase in phospho-JNK expression, a 2.2-fold increase in phospho-p38, and 1.9-fold increase in phospho-ERK expression. There were no significant differences in the early-activated LV septum between paced and sham dogs. Conclusions: Temporal dispersion of mechanical activation by RV apical pacing induced spatial dispersion of protein expression in the LV.

KW - calcium handling protein

KW - Dyssynchrony

KW - heart failure

KW - mitogen-activated protein kinase

KW - ventricular pacing

UR - http://www.scopus.com/inward/record.url?scp=77955509811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955509811&partnerID=8YFLogxK

U2 - 10.1016/j.cardfail.2010.04.001

DO - 10.1016/j.cardfail.2010.04.001

M3 - Article

C2 - 20670849

AN - SCOPUS:77955509811

VL - 16

SP - 700

EP - 706

JO - Journal of Cardiac Failure

JF - Journal of Cardiac Failure

SN - 1071-9164

IS - 8

ER -