Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis

Chia Yang Hung, Tsung I. Hsu, Jian Ying Chuang, Tsung Ping Su, Wen Chang Chang, Jan Jong Hung

Research output: Contribution to journalArticle

Abstract

In this study, we found that Sp1 was highly expressed in astrocytes, implying that Sp1 might be important for the function of astrocytes. Sp1/GFAP-Cre-ERT2 conditional knockout mice were constructed to study the role of Sp1 in astrocytes. Knockout of Sp1 in astrocytes altered astrocytic morphology and decreased GFAP expression in the cortex and hippocampus but did not affect cell viability. Loss of Sp1 in astrocytes decreased the number of neurons in the cortex and hippocampus. Conditioned medium from primary astrocytes with Sp1 knockout disrupted neuronal dendritic outgrowth and synapse formation, resulting in abnormal learning, memory, and motor behavior. Sp1 knockout in astrocytes altered gene expression, including decreasing the expression of Toll-like receptor 2 and Cfb and increasing the expression of C1q and C4Bp, thereby affecting neurite outgrowth and synapse formation, resulting in disordered neuron function. Studying these gene regulations might be beneficial to understanding neuronal development and brain injury prevention.

Original languageEnglish
JournalMolecular Neurobiology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Astrocytes
Synapses
Hippocampus
Neurons
Toll-Like Receptor 2
Conditioned Culture Medium
Neuronal Outgrowth
Knockout Mice
Brain Injuries
Cell Survival
Learning
Gene Expression
Genes

Keywords

  • Astrocyte
  • Neurite outgrowth
  • Sp1
  • Synaptogenesis

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis. / Hung, Chia Yang; Hsu, Tsung I.; Chuang, Jian Ying; Su, Tsung Ping; Chang, Wen Chang; Hung, Jan Jong.

In: Molecular Neurobiology, 01.01.2019.

Research output: Contribution to journalArticle

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