Soot-driven reactive oxygen species formation from incense burning

Hsiao Chi Chuang, Tim P. Jones, Shih Chun C Lung, Kelly A. BéruBé

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This study investigated the effects of reactive oxygen species (ROS) generated as a function of the physicochemistry of incense particulate matter (IPM), diesel exhaust particles (DEP) and carbon black (CB). Microscopical and elemental analyses were used to determine particle morphology and inorganic compounds. ROS was determined using the reactive dye, Dichlorodihydrofluorescin (DCFH), and the Plasmid Scission Assay (PSA), which determine DNA damage. Two common types of soot were observed within IPM, including nano-soot and micro-soot, whereas DEP and CB mainly consisted of nano-soot. These PM were capable of causing oxidative stress in a dose-dependent manner, especially IPM and DEP. A dose of IPM (36.6-102.3. μg/ml) was capable of causing 50% oxidative DNA damage. ROS formation was positively correlated to smaller nano-soot aggregates and bulk metallic compounds, particularly Cu. These observations have important implications for respiratory health given that inflammation has been recognised as an important factor in the development of lung injury/diseases by oxidative stress. This study supports the view that ROS formation by combustion-derived PM is related to PM physicochemistry, and also provides new data for IPM.

Original languageEnglish
Pages (from-to)4781-4787
Number of pages7
JournalScience of the Total Environment
Volume409
Issue number22
DOIs
Publication statusPublished - Oct 15 2011
Externally publishedYes

Fingerprint

Soot
soot
particulate matter
Reactive Oxygen Species
Particulate Matter
Oxygen
Vehicle Emissions
diesel
Oxidative stress
black carbon
Carbon black
DNA
Metallic compounds
Inorganic compounds
Pulmonary diseases
inorganic compound
damage
plasmid
Assays
dye

Keywords

  • CB
  • DEP
  • Incense
  • Physicochemistry
  • ROS
  • Soot

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering

Cite this

Soot-driven reactive oxygen species formation from incense burning. / Chuang, Hsiao Chi; Jones, Tim P.; Lung, Shih Chun C; BéruBé, Kelly A.

In: Science of the Total Environment, Vol. 409, No. 22, 15.10.2011, p. 4781-4787.

Research output: Contribution to journalArticle

Chuang, Hsiao Chi ; Jones, Tim P. ; Lung, Shih Chun C ; BéruBé, Kelly A. / Soot-driven reactive oxygen species formation from incense burning. In: Science of the Total Environment. 2011 ; Vol. 409, No. 22. pp. 4781-4787.
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