Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

Chung Ren Lin, Kuan Hung Chen, Chien Hui Yang, Jiin Tsuey Cheng, Shyr Ming Sheen-Chen, Chih Hsien Wu, Wei Dih Sy, Yi Shen Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

Original languageEnglish
Article number44
JournalJournal of Biomedical Science
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells'. Together they form a unique fingerprint.

  • Cite this

    Lin, C. R., Chen, K. H., Yang, C. H., Cheng, J. T., Sheen-Chen, S. M., Wu, C. H., Sy, W. D., & Chen, Y. S. (2010). Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells. Journal of Biomedical Science, 17(1), [44]. https://doi.org/10.1186/1423-0127-17-44