Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

Chung Ren Lin, Kuan Hung Chen, Chien Hui Yang, Jiin Tsuey Cheng, Shyr Ming Sheen-Chen, Chih Hsien Wu, Wei Dih Sy, Yi Shen Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

Original languageEnglish
Article number44
JournalJournal of Biomedical Science
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

Fingerprint

Gene transfer
Spinal Ganglia
Neurons
Transfection
Rats
Genes
Ultrasonics
Gene Transfer Techniques
Cell death
Liposomes
Cell Survival
Cell Death
Cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells. / Lin, Chung Ren; Chen, Kuan Hung; Yang, Chien Hui; Cheng, Jiin Tsuey; Sheen-Chen, Shyr Ming; Wu, Chih Hsien; Sy, Wei Dih; Chen, Yi Shen.

In: Journal of Biomedical Science, Vol. 17, No. 1, 44, 01.01.2010.

Research output: Contribution to journalArticle

Lin, Chung Ren ; Chen, Kuan Hung ; Yang, Chien Hui ; Cheng, Jiin Tsuey ; Sheen-Chen, Shyr Ming ; Wu, Chih Hsien ; Sy, Wei Dih ; Chen, Yi Shen. / Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells. In: Journal of Biomedical Science. 2010 ; Vol. 17, No. 1.
@article{0d0f2f7773ec4f82b7a585ec8f70b1f3,
title = "Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells",
abstract = "Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31{\%} and a survival rate of 35{\%}. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.",
author = "Lin, {Chung Ren} and Chen, {Kuan Hung} and Yang, {Chien Hui} and Cheng, {Jiin Tsuey} and Sheen-Chen, {Shyr Ming} and Wu, {Chih Hsien} and Sy, {Wei Dih} and Chen, {Yi Shen}",
year = "2010",
month = "1",
day = "1",
doi = "10.1186/1423-0127-17-44",
language = "English",
volume = "17",
journal = "Journal of Biomedical Science",
issn = "1021-7770",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

AU - Lin, Chung Ren

AU - Chen, Kuan Hung

AU - Yang, Chien Hui

AU - Cheng, Jiin Tsuey

AU - Sheen-Chen, Shyr Ming

AU - Wu, Chih Hsien

AU - Sy, Wei Dih

AU - Chen, Yi Shen

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

AB - Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

UR - http://www.scopus.com/inward/record.url?scp=77952968365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952968365&partnerID=8YFLogxK

U2 - 10.1186/1423-0127-17-44

DO - 10.1186/1423-0127-17-44

M3 - Article

C2 - 20525259

AN - SCOPUS:77952968365

VL - 17

JO - Journal of Biomedical Science

JF - Journal of Biomedical Science

SN - 1021-7770

IS - 1

M1 - 44

ER -