Soluble CD44 isoforms in serum as potential markers of metastatic gastric carcinoma

Horng Jyh Harn, Li Ing Ho, Rong Yaun Shyu, Jiang Shun Yuan, Fu Gong Lin, Ton Ho Young, Ching Ann Liu, Hung Shang Tang, Wei Hwa Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A splice variant of CD44 (exon V4-V7) confers metastatic behavior in a rat carcinoma model; aberrant expression of splice variants has been detected on a variety of human tumor cell lines as well as primary and metastatic human tumors, including lymphomas, carcinomas (colon, thyroid, mamma, bladder), and glioma. We used enzyme-linked immunosorbent assay to determine the concentration of soluble CD44 in the serum samples of 10 normal individuals and 41 patients with various stages of gastric cancer. Soluble CD44S and its isoforms, V5 and V6, were present in the serum of normal individuals (288.53 ± 18.33, 25.49 ± 1.70, and 148.32 ± 3.15 ng/ml, respectively). The concentrations of soluble CD44 V5 and V6 were elevated in patients with advanced gastric carcinoma (69.39 ± 6.06 and 216.62 ± 32.98 ng/ml, respectively). Serum CD44 V5 concentrations correlated with the extent of tumor invasion (T), the status of lymph node involvement (N), and distant metastasis (M) (TNM staging) (p <0.05), whereas CD44S did not. These results suggest that detection of abnormal regulation of CD44 splicing could be helpful in gastric cancer diagnosis and disease evaluation.

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalJournal of Clinical Gastroenterology
Volume22
Issue number2
DOIs
Publication statusPublished - Mar 1996
Externally publishedYes

Keywords

  • Enzyme-linked immunosorbent assay
  • Soluble CD44
  • Stomach adenocarcinoma

ASJC Scopus subject areas

  • Gastroenterology

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  • Cite this

    Harn, H. J., Ho, L. I., Shyu, R. Y., Yuan, J. S., Lin, F. G., Young, T. H., Liu, C. A., Tang, H. S., & Lee, W. H. (1996). Soluble CD44 isoforms in serum as potential markers of metastatic gastric carcinoma. Journal of Clinical Gastroenterology, 22(2), 107-110. https://doi.org/10.1097/00004836-199603000-00006