Slow Freezing Coupled Static Magnetic Field Exposure Enhances Cryopreservative Efficiency-A Study on Human Erythrocytes

Chun Yen Lin, Po Li Wei, Wei Jen Chang, Yung Kai Huang, Sheng-Wei Feng, Che Tong Lin, Sheng Yang Lee, Haw Ming Huang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The aim of this study was to assess the cryoprotective effect of static magnetic fields (SMFs) on human erythrocytes during the slow cooling procedure. Human erythrocytes suspended in 20% glycerol were slowly frozen with a 0.4-T or 0.8-T SMF and then moved to a -80°C freezer for 24 hr. The changes in survival rate, morphology, and metabolites of the thawed erythrocytes were examined. To understand possible cryoprotective mechanisms of SMF, membrane fluidity and dehydration stability of SMF-exposed erythrocytes were tested. For each test, sham-exposed erythrocytes were used as controls. Our results showed that freezing coupled with 0.4-T or 0.8-T SMFs significantly increased the relative survival ratios of the frozen-thawed erythrocytes by 10% and 20% (p

Original languageEnglish
Article numbere58988
JournalPLoS One
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 8 2013

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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