Size, subunit composition, and secondary structure of the Friend virus genome

S. Dube, H. J. Kung, W. Bender, N. Davidson, W. Ostertag

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Electron microscope and gel electrophoresis studies show that the high molecular weight (50 to 70S) RNA extracted from Friend virus (FV) is a dimer with the same basic structure previously observed for the RNAs from RD 114 virus, baboon virus, and woolly monkey virus. This observation greatly strengthens the inference that the dimer structure is a general characteristic of the RNAs of all mammalian type C viruses. The FV dimer is slightly less stable than the RNA dimer of woolly monkey virus, which is, in turn, much less stable than those of RD 114 and baboon virus. There are three FV monomer components, small (S), medium (M), and large (L), with molecular lengths of 6.7 ± 0.6, 7.7 ± 0.6, and 9.5 ± 0.6 kilobases, respectively. There are approximately equal amounts of the S and M components and much less of the L component. Most of the dimers are homodimers (SS, MM, and LL). The frequency of heterodimers (SM, SL, ML) is much less than expected for a random assortment model.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalJournal of Virology
Volume20
Issue number1
Publication statusPublished - Dec 1 1976
Externally publishedYes

Fingerprint

Friend murine leukemia virus
Woolly Monkey Sarcoma Virus
Genome
RNA
Viruses
viruses
genome
Papio
Myeloma Proteins
Viral Structures
Electrophoresis
monkeys
Molecular Weight
Gels
Electrons
electron microscopes
gel electrophoresis
molecular weight

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Dube, S., Kung, H. J., Bender, W., Davidson, N., & Ostertag, W. (1976). Size, subunit composition, and secondary structure of the Friend virus genome. Journal of Virology, 20(1), 264-272.

Size, subunit composition, and secondary structure of the Friend virus genome. / Dube, S.; Kung, H. J.; Bender, W.; Davidson, N.; Ostertag, W.

In: Journal of Virology, Vol. 20, No. 1, 01.12.1976, p. 264-272.

Research output: Contribution to journalArticle

Dube, S, Kung, HJ, Bender, W, Davidson, N & Ostertag, W 1976, 'Size, subunit composition, and secondary structure of the Friend virus genome', Journal of Virology, vol. 20, no. 1, pp. 264-272.
Dube S, Kung HJ, Bender W, Davidson N, Ostertag W. Size, subunit composition, and secondary structure of the Friend virus genome. Journal of Virology. 1976 Dec 1;20(1):264-272.
Dube, S. ; Kung, H. J. ; Bender, W. ; Davidson, N. ; Ostertag, W. / Size, subunit composition, and secondary structure of the Friend virus genome. In: Journal of Virology. 1976 ; Vol. 20, No. 1. pp. 264-272.
@article{b64811a8c03c472e8203919bd66dfe9b,
title = "Size, subunit composition, and secondary structure of the Friend virus genome",
abstract = "Electron microscope and gel electrophoresis studies show that the high molecular weight (50 to 70S) RNA extracted from Friend virus (FV) is a dimer with the same basic structure previously observed for the RNAs from RD 114 virus, baboon virus, and woolly monkey virus. This observation greatly strengthens the inference that the dimer structure is a general characteristic of the RNAs of all mammalian type C viruses. The FV dimer is slightly less stable than the RNA dimer of woolly monkey virus, which is, in turn, much less stable than those of RD 114 and baboon virus. There are three FV monomer components, small (S), medium (M), and large (L), with molecular lengths of 6.7 ± 0.6, 7.7 ± 0.6, and 9.5 ± 0.6 kilobases, respectively. There are approximately equal amounts of the S and M components and much less of the L component. Most of the dimers are homodimers (SS, MM, and LL). The frequency of heterodimers (SM, SL, ML) is much less than expected for a random assortment model.",
author = "S. Dube and Kung, {H. J.} and W. Bender and N. Davidson and W. Ostertag",
year = "1976",
month = "12",
day = "1",
language = "English",
volume = "20",
pages = "264--272",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Size, subunit composition, and secondary structure of the Friend virus genome

AU - Dube, S.

AU - Kung, H. J.

AU - Bender, W.

AU - Davidson, N.

AU - Ostertag, W.

PY - 1976/12/1

Y1 - 1976/12/1

N2 - Electron microscope and gel electrophoresis studies show that the high molecular weight (50 to 70S) RNA extracted from Friend virus (FV) is a dimer with the same basic structure previously observed for the RNAs from RD 114 virus, baboon virus, and woolly monkey virus. This observation greatly strengthens the inference that the dimer structure is a general characteristic of the RNAs of all mammalian type C viruses. The FV dimer is slightly less stable than the RNA dimer of woolly monkey virus, which is, in turn, much less stable than those of RD 114 and baboon virus. There are three FV monomer components, small (S), medium (M), and large (L), with molecular lengths of 6.7 ± 0.6, 7.7 ± 0.6, and 9.5 ± 0.6 kilobases, respectively. There are approximately equal amounts of the S and M components and much less of the L component. Most of the dimers are homodimers (SS, MM, and LL). The frequency of heterodimers (SM, SL, ML) is much less than expected for a random assortment model.

AB - Electron microscope and gel electrophoresis studies show that the high molecular weight (50 to 70S) RNA extracted from Friend virus (FV) is a dimer with the same basic structure previously observed for the RNAs from RD 114 virus, baboon virus, and woolly monkey virus. This observation greatly strengthens the inference that the dimer structure is a general characteristic of the RNAs of all mammalian type C viruses. The FV dimer is slightly less stable than the RNA dimer of woolly monkey virus, which is, in turn, much less stable than those of RD 114 and baboon virus. There are three FV monomer components, small (S), medium (M), and large (L), with molecular lengths of 6.7 ± 0.6, 7.7 ± 0.6, and 9.5 ± 0.6 kilobases, respectively. There are approximately equal amounts of the S and M components and much less of the L component. Most of the dimers are homodimers (SS, MM, and LL). The frequency of heterodimers (SM, SL, ML) is much less than expected for a random assortment model.

UR - http://www.scopus.com/inward/record.url?scp=0017108246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017108246&partnerID=8YFLogxK

M3 - Article

C2 - 978793

AN - SCOPUS:0017108246

VL - 20

SP - 264

EP - 272

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -