Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles

Fang Lu, Si Han Wu, Yann Hung, Chung Yuan Mou

Research output: Contribution to journalArticle

611 Citations (Scopus)

Abstract

A study was conducted to demonstrate the synthesis of monodisperse mesoporous silica nanoparticle (MSN) suspensions and investigate the effect of nanoparticle size on cell uptake. The MSNs were synthesized by adjusting the pH of the reaction medium, leading to a series of MSNs with uniform size ranging from 30-280 nm. The physical properties of the MSNs were characterized with various methods, such as nitrogen adsorption-desorption, transmission electron microscopy (TEM), and X-ray powder diffraction (XRD). The particle size effect on the cell uptake efficiency with human cervical cancer cells and cellular uptake of MSNs of various sizes were investigated by confocal laser scanning microscopy (CLSM). The uptake efficiencies were determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis of total silicon content.

Original languageEnglish
Pages (from-to)1408-1413
Number of pages6
JournalSmall
Volume5
Issue number12
DOIs
Publication statusPublished - Jun 19 2009
Externally publishedYes

Fingerprint

Silicon Dioxide
Nanoparticles
Silica
Powder Diffraction
Inductively coupled plasma mass spectrometry
Silicon
Transmission Electron Microscopy
Cell Size
Particle Size
Confocal Microscopy
X-Ray Diffraction
Uterine Cervical Neoplasms
X ray powder diffraction
Adsorption
Desorption
Mass Spectrometry
Suspensions
Microscopic examination
Nitrogen
Physical properties

Keywords

  • Cell uptake
  • Mesoporous materials
  • Nanoparticles
  • Particle size
  • Silica

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology

Cite this

Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles. / Lu, Fang; Wu, Si Han; Hung, Yann; Mou, Chung Yuan.

In: Small, Vol. 5, No. 12, 19.06.2009, p. 1408-1413.

Research output: Contribution to journalArticle

Lu, Fang ; Wu, Si Han ; Hung, Yann ; Mou, Chung Yuan. / Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles. In: Small. 2009 ; Vol. 5, No. 12. pp. 1408-1413.
@article{2bfe8dde0b5e46228a507e7f7d0d55e5,
title = "Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles",
abstract = "A study was conducted to demonstrate the synthesis of monodisperse mesoporous silica nanoparticle (MSN) suspensions and investigate the effect of nanoparticle size on cell uptake. The MSNs were synthesized by adjusting the pH of the reaction medium, leading to a series of MSNs with uniform size ranging from 30-280 nm. The physical properties of the MSNs were characterized with various methods, such as nitrogen adsorption-desorption, transmission electron microscopy (TEM), and X-ray powder diffraction (XRD). The particle size effect on the cell uptake efficiency with human cervical cancer cells and cellular uptake of MSNs of various sizes were investigated by confocal laser scanning microscopy (CLSM). The uptake efficiencies were determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis of total silicon content.",
keywords = "Cell uptake, Mesoporous materials, Nanoparticles, Particle size, Silica",
author = "Fang Lu and Wu, {Si Han} and Yann Hung and Mou, {Chung Yuan}",
year = "2009",
month = "6",
day = "19",
doi = "10.1002/smll.200900005",
language = "English",
volume = "5",
pages = "1408--1413",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
number = "12",

}

TY - JOUR

T1 - Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles

AU - Lu, Fang

AU - Wu, Si Han

AU - Hung, Yann

AU - Mou, Chung Yuan

PY - 2009/6/19

Y1 - 2009/6/19

N2 - A study was conducted to demonstrate the synthesis of monodisperse mesoporous silica nanoparticle (MSN) suspensions and investigate the effect of nanoparticle size on cell uptake. The MSNs were synthesized by adjusting the pH of the reaction medium, leading to a series of MSNs with uniform size ranging from 30-280 nm. The physical properties of the MSNs were characterized with various methods, such as nitrogen adsorption-desorption, transmission electron microscopy (TEM), and X-ray powder diffraction (XRD). The particle size effect on the cell uptake efficiency with human cervical cancer cells and cellular uptake of MSNs of various sizes were investigated by confocal laser scanning microscopy (CLSM). The uptake efficiencies were determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis of total silicon content.

AB - A study was conducted to demonstrate the synthesis of monodisperse mesoporous silica nanoparticle (MSN) suspensions and investigate the effect of nanoparticle size on cell uptake. The MSNs were synthesized by adjusting the pH of the reaction medium, leading to a series of MSNs with uniform size ranging from 30-280 nm. The physical properties of the MSNs were characterized with various methods, such as nitrogen adsorption-desorption, transmission electron microscopy (TEM), and X-ray powder diffraction (XRD). The particle size effect on the cell uptake efficiency with human cervical cancer cells and cellular uptake of MSNs of various sizes were investigated by confocal laser scanning microscopy (CLSM). The uptake efficiencies were determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis of total silicon content.

KW - Cell uptake

KW - Mesoporous materials

KW - Nanoparticles

KW - Particle size

KW - Silica

UR - http://www.scopus.com/inward/record.url?scp=67650338048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650338048&partnerID=8YFLogxK

U2 - 10.1002/smll.200900005

DO - 10.1002/smll.200900005

M3 - Article

C2 - 19296554

AN - SCOPUS:67650338048

VL - 5

SP - 1408

EP - 1413

JO - Small

JF - Small

SN - 1613-6810

IS - 12

ER -