Abstract
Chemotaxis of cancer cells is an essential component of tumor dissemination. The chemotactic response is comprised of three separate steps, including chemosensing, polarization and locomotion. We present an innovative approach on chemotaxis assay to address cancer cell chemotaxis. We applied a high-resolution optical tweezers system to manipulate epidermal growth factor (EGF)-coated beads positioned close to the filopodia, to locally stimulate HT29 cells expressing the EGF receptor (EGFR). We demonstrated that membrane protrusion at the leading edge induced by an EGF chemotaxis occurred at about 30∼40 s. In addition, the present observation revealed that the locomotion of HT29 cell depended on whether the HT29 cell sensed the presence of the chemoattractant EGF. We anticipate the proposed approach based on optical tweezers, together with the platform at single-cell level, could be applied to build a quick screening method for detection and treatment evaluation of many types of cancer during chemotaxis.
| Original language | English |
|---|---|
| Pages (from-to) | 259-265 |
| Number of pages | 7 |
| Journal | Journal of Polymer Engineering |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 1 2014 |
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Keywords
- chemosensing
- chemotaxis
- epidermal growth factor
- locomotion
- optical tweezers
ASJC Scopus subject areas
- Materials Chemistry
- Polymers and Plastics
- Chemical Engineering(all)
Cite this
Single-molecule manipulation and detection platform for studying cancer cell chemotaxis. / Chen, May Show; Peng, Pei Wen; Liou, Bing Chun; Kuo, Hsiao Chen; Ou, Keng Liang; Yang, Tzu Sen.
In: Journal of Polymer Engineering, Vol. 34, No. 3, 01.05.2014, p. 259-265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-molecule manipulation and detection platform for studying cancer cell chemotaxis
AU - Chen, May Show
AU - Peng, Pei Wen
AU - Liou, Bing Chun
AU - Kuo, Hsiao Chen
AU - Ou, Keng Liang
AU - Yang, Tzu Sen
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Chemotaxis of cancer cells is an essential component of tumor dissemination. The chemotactic response is comprised of three separate steps, including chemosensing, polarization and locomotion. We present an innovative approach on chemotaxis assay to address cancer cell chemotaxis. We applied a high-resolution optical tweezers system to manipulate epidermal growth factor (EGF)-coated beads positioned close to the filopodia, to locally stimulate HT29 cells expressing the EGF receptor (EGFR). We demonstrated that membrane protrusion at the leading edge induced by an EGF chemotaxis occurred at about 30∼40 s. In addition, the present observation revealed that the locomotion of HT29 cell depended on whether the HT29 cell sensed the presence of the chemoattractant EGF. We anticipate the proposed approach based on optical tweezers, together with the platform at single-cell level, could be applied to build a quick screening method for detection and treatment evaluation of many types of cancer during chemotaxis.
AB - Chemotaxis of cancer cells is an essential component of tumor dissemination. The chemotactic response is comprised of three separate steps, including chemosensing, polarization and locomotion. We present an innovative approach on chemotaxis assay to address cancer cell chemotaxis. We applied a high-resolution optical tweezers system to manipulate epidermal growth factor (EGF)-coated beads positioned close to the filopodia, to locally stimulate HT29 cells expressing the EGF receptor (EGFR). We demonstrated that membrane protrusion at the leading edge induced by an EGF chemotaxis occurred at about 30∼40 s. In addition, the present observation revealed that the locomotion of HT29 cell depended on whether the HT29 cell sensed the presence of the chemoattractant EGF. We anticipate the proposed approach based on optical tweezers, together with the platform at single-cell level, could be applied to build a quick screening method for detection and treatment evaluation of many types of cancer during chemotaxis.
KW - chemosensing
KW - chemotaxis
KW - epidermal growth factor
KW - locomotion
KW - optical tweezers
UR - http://www.scopus.com/inward/record.url?scp=84899809114&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899809114&partnerID=8YFLogxK
U2 - 10.1515/polyeng-2013-0217
DO - 10.1515/polyeng-2013-0217
M3 - Article
AN - SCOPUS:84899809114
VL - 34
SP - 259
EP - 265
JO - Journal of Polymer Engineering
JF - Journal of Polymer Engineering
SN - 0334-6447
IS - 3
ER -