Single-layer periosteum progenitor cell sheet significantly promotes tendon-bone healing in comparison with acellular collagen sheet in anterior cruciate ligament reconstruction

Lei Yen Chen, Shu Wen Whu, Chih Hwa Chen, Hsia Wei Liu, Hsien Tao Liu, Chih Hsiang Chang, Ching Lin Tsai, Shih Hui Chen, Ging Ho Hsiue

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Successful tendon-bone healing in anterior cruciate ligament reconstruction can be achieved by introducing a suitable medium into the tendon-bone junction. Two new feasible media, periosteum progenitor cell sheet (PPCS) and acellular collagen sheet (ACS), were utilized to promote tendon-bone healing. This study aimed to compare the effects of these two media on tendon-bone healing in a rabbit model. Methods: Periosteum progenitor cells were harvested from rabbit tibial periosteum, cultivated on polymerized fibrin-coated dishes, and obtained as PPCS. ACS was prepared from rabbit small intestinal submucosa. Twenty rabbits were used in this study. Tendon grafts wrapped in cell sheet were pulled into a bone tunnel in a model of anterior cruciate ligament reconstruction. Rabbits were sacrificed at 8 weeks postoperatively for histological, immunohistochemical, and biomechanical assay. Results: The monolayer PPCS that had been wrapped around the tendon before surgery was analysed. Histological staining revealed that PPCS gave rise to higher collagen and glycosaminoglycan deposition with fibrocartilage formation than ACS at the tendon-bone junction at 8 weeks. In addition, PPCS produced significantly better mechanical results compared with ACS, including increased maximum load, stiffness, and maximum load per length of bone tunnel. Conclusions: Single-layer PPCS significantly enhanced tendon-bone healing compared with ACS, with fibrocartilage formation at the tendon interface and superior biomechanical strength.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalFormosan Journal of Musculoskeletal Disorders
Volume3
Issue number1
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

Fingerprint

Periosteum
Anterior Cruciate Ligament Reconstruction
Tendons
Collagen
Stem Cells
Bone and Bones
Rabbits
Fibrocartilage
Glycosaminoglycans
Fibrin
Staining and Labeling
Transplants

Keywords

  • Anterior cruciate ligament reconstruction
  • Periosteum progenitor cell sheet
  • Tendon bone healing

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Single-layer periosteum progenitor cell sheet significantly promotes tendon-bone healing in comparison with acellular collagen sheet in anterior cruciate ligament reconstruction. / Chen, Lei Yen; Whu, Shu Wen; Chen, Chih Hwa; Liu, Hsia Wei; Liu, Hsien Tao; Chang, Chih Hsiang; Tsai, Ching Lin; Chen, Shih Hui; Hsiue, Ging Ho.

In: Formosan Journal of Musculoskeletal Disorders, Vol. 3, No. 1, 02.2012, p. 1-8.

Research output: Contribution to journalArticle

Chen, Lei Yen ; Whu, Shu Wen ; Chen, Chih Hwa ; Liu, Hsia Wei ; Liu, Hsien Tao ; Chang, Chih Hsiang ; Tsai, Ching Lin ; Chen, Shih Hui ; Hsiue, Ging Ho. / Single-layer periosteum progenitor cell sheet significantly promotes tendon-bone healing in comparison with acellular collagen sheet in anterior cruciate ligament reconstruction. In: Formosan Journal of Musculoskeletal Disorders. 2012 ; Vol. 3, No. 1. pp. 1-8.
@article{d3dfdbbb75a04d718d2846d273c3dd84,
title = "Single-layer periosteum progenitor cell sheet significantly promotes tendon-bone healing in comparison with acellular collagen sheet in anterior cruciate ligament reconstruction",
abstract = "Purpose: Successful tendon-bone healing in anterior cruciate ligament reconstruction can be achieved by introducing a suitable medium into the tendon-bone junction. Two new feasible media, periosteum progenitor cell sheet (PPCS) and acellular collagen sheet (ACS), were utilized to promote tendon-bone healing. This study aimed to compare the effects of these two media on tendon-bone healing in a rabbit model. Methods: Periosteum progenitor cells were harvested from rabbit tibial periosteum, cultivated on polymerized fibrin-coated dishes, and obtained as PPCS. ACS was prepared from rabbit small intestinal submucosa. Twenty rabbits were used in this study. Tendon grafts wrapped in cell sheet were pulled into a bone tunnel in a model of anterior cruciate ligament reconstruction. Rabbits were sacrificed at 8 weeks postoperatively for histological, immunohistochemical, and biomechanical assay. Results: The monolayer PPCS that had been wrapped around the tendon before surgery was analysed. Histological staining revealed that PPCS gave rise to higher collagen and glycosaminoglycan deposition with fibrocartilage formation than ACS at the tendon-bone junction at 8 weeks. In addition, PPCS produced significantly better mechanical results compared with ACS, including increased maximum load, stiffness, and maximum load per length of bone tunnel. Conclusions: Single-layer PPCS significantly enhanced tendon-bone healing compared with ACS, with fibrocartilage formation at the tendon interface and superior biomechanical strength.",
keywords = "Anterior cruciate ligament reconstruction, Periosteum progenitor cell sheet, Tendon bone healing",
author = "Chen, {Lei Yen} and Whu, {Shu Wen} and Chen, {Chih Hwa} and Liu, {Hsia Wei} and Liu, {Hsien Tao} and Chang, {Chih Hsiang} and Tsai, {Ching Lin} and Chen, {Shih Hui} and Hsiue, {Ging Ho}",
year = "2012",
month = "2",
doi = "10.1016/j.fjmd.2012.01.001",
language = "English",
volume = "3",
pages = "1--8",
journal = "Formosan Journal of Musculoskeletal Disorders",
issn = "2210-7940",
publisher = "中華民國骨科醫學會",
number = "1",

}

TY - JOUR

T1 - Single-layer periosteum progenitor cell sheet significantly promotes tendon-bone healing in comparison with acellular collagen sheet in anterior cruciate ligament reconstruction

AU - Chen, Lei Yen

AU - Whu, Shu Wen

AU - Chen, Chih Hwa

AU - Liu, Hsia Wei

AU - Liu, Hsien Tao

AU - Chang, Chih Hsiang

AU - Tsai, Ching Lin

AU - Chen, Shih Hui

AU - Hsiue, Ging Ho

PY - 2012/2

Y1 - 2012/2

N2 - Purpose: Successful tendon-bone healing in anterior cruciate ligament reconstruction can be achieved by introducing a suitable medium into the tendon-bone junction. Two new feasible media, periosteum progenitor cell sheet (PPCS) and acellular collagen sheet (ACS), were utilized to promote tendon-bone healing. This study aimed to compare the effects of these two media on tendon-bone healing in a rabbit model. Methods: Periosteum progenitor cells were harvested from rabbit tibial periosteum, cultivated on polymerized fibrin-coated dishes, and obtained as PPCS. ACS was prepared from rabbit small intestinal submucosa. Twenty rabbits were used in this study. Tendon grafts wrapped in cell sheet were pulled into a bone tunnel in a model of anterior cruciate ligament reconstruction. Rabbits were sacrificed at 8 weeks postoperatively for histological, immunohistochemical, and biomechanical assay. Results: The monolayer PPCS that had been wrapped around the tendon before surgery was analysed. Histological staining revealed that PPCS gave rise to higher collagen and glycosaminoglycan deposition with fibrocartilage formation than ACS at the tendon-bone junction at 8 weeks. In addition, PPCS produced significantly better mechanical results compared with ACS, including increased maximum load, stiffness, and maximum load per length of bone tunnel. Conclusions: Single-layer PPCS significantly enhanced tendon-bone healing compared with ACS, with fibrocartilage formation at the tendon interface and superior biomechanical strength.

AB - Purpose: Successful tendon-bone healing in anterior cruciate ligament reconstruction can be achieved by introducing a suitable medium into the tendon-bone junction. Two new feasible media, periosteum progenitor cell sheet (PPCS) and acellular collagen sheet (ACS), were utilized to promote tendon-bone healing. This study aimed to compare the effects of these two media on tendon-bone healing in a rabbit model. Methods: Periosteum progenitor cells were harvested from rabbit tibial periosteum, cultivated on polymerized fibrin-coated dishes, and obtained as PPCS. ACS was prepared from rabbit small intestinal submucosa. Twenty rabbits were used in this study. Tendon grafts wrapped in cell sheet were pulled into a bone tunnel in a model of anterior cruciate ligament reconstruction. Rabbits were sacrificed at 8 weeks postoperatively for histological, immunohistochemical, and biomechanical assay. Results: The monolayer PPCS that had been wrapped around the tendon before surgery was analysed. Histological staining revealed that PPCS gave rise to higher collagen and glycosaminoglycan deposition with fibrocartilage formation than ACS at the tendon-bone junction at 8 weeks. In addition, PPCS produced significantly better mechanical results compared with ACS, including increased maximum load, stiffness, and maximum load per length of bone tunnel. Conclusions: Single-layer PPCS significantly enhanced tendon-bone healing compared with ACS, with fibrocartilage formation at the tendon interface and superior biomechanical strength.

KW - Anterior cruciate ligament reconstruction

KW - Periosteum progenitor cell sheet

KW - Tendon bone healing

UR - http://www.scopus.com/inward/record.url?scp=84862788940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862788940&partnerID=8YFLogxK

U2 - 10.1016/j.fjmd.2012.01.001

DO - 10.1016/j.fjmd.2012.01.001

M3 - Article

VL - 3

SP - 1

EP - 8

JO - Formosan Journal of Musculoskeletal Disorders

JF - Formosan Journal of Musculoskeletal Disorders

SN - 2210-7940

IS - 1

ER -