Simultaneous measurement of urinary ketamine, norketamine, and dehydronorketamine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

Gong-Jhe Wu, Han Chan, Maw Rong Lee, Chung Yu Chen, Dar Yu Yang, Fu Chou Cheng

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A liquid chromatography/atmospheric pressure ionization mass spectrometry has been developed for the determination of ketamine, norketamine, and dehydronorketamine in human urine. A separation of these analytes in urine samples without tedious pretreatment procedures has been achieved within 10 min. Linear calibration curves of these analytes with coefficients better than 0.998 have been obtained over a wide range from 12.5 to 200 ng/mL. The accuracy was between 2.1% and 7.2% with detection limits at levels of 0.02 ng/mL, 0.02 ng/mL and 0.93 ng/mL for ketamine, norketamine and dehydronorketamine, respectively. The results demonstrate the suitability of the liquid chromatography/atmospheric pressure ionization mass spectrometry approach to analyze trace ketamine, norketamine and dehydronorketamine in urine. Urinary ketamine and norketamine levels were relatively low at 4-24 h intervals and were difficult to assay in a normal laboratory. In the present study, the determination of urinary dehydronorketamine levels at 2-24 hours appears to have a great potential for use in Schedule III controlled drugs management.

Original languageEnglish
Pages (from-to)351-355
Number of pages5
JournalJournal of the Chinese Chemical Society
Volume54
Issue number2
Publication statusPublished - 2007

Fingerprint

Liquid chromatography
Ketamine
Atmospheric pressure
Ionization
Mass spectrometry
norketamine
Assays
Calibration
Pharmaceutical Preparations

Keywords

  • APCI
  • Dehydronorketamine
  • Ketamine
  • LC
  • MS
  • Norketamine
  • Urine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Simultaneous measurement of urinary ketamine, norketamine, and dehydronorketamine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. / Wu, Gong-Jhe; Chan, Han; Lee, Maw Rong; Chen, Chung Yu; Yang, Dar Yu; Cheng, Fu Chou.

In: Journal of the Chinese Chemical Society, Vol. 54, No. 2, 2007, p. 351-355.

Research output: Contribution to journalArticle

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T1 - Simultaneous measurement of urinary ketamine, norketamine, and dehydronorketamine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

AU - Wu, Gong-Jhe

AU - Chan, Han

AU - Lee, Maw Rong

AU - Chen, Chung Yu

AU - Yang, Dar Yu

AU - Cheng, Fu Chou

PY - 2007

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N2 - A liquid chromatography/atmospheric pressure ionization mass spectrometry has been developed for the determination of ketamine, norketamine, and dehydronorketamine in human urine. A separation of these analytes in urine samples without tedious pretreatment procedures has been achieved within 10 min. Linear calibration curves of these analytes with coefficients better than 0.998 have been obtained over a wide range from 12.5 to 200 ng/mL. The accuracy was between 2.1% and 7.2% with detection limits at levels of 0.02 ng/mL, 0.02 ng/mL and 0.93 ng/mL for ketamine, norketamine and dehydronorketamine, respectively. The results demonstrate the suitability of the liquid chromatography/atmospheric pressure ionization mass spectrometry approach to analyze trace ketamine, norketamine and dehydronorketamine in urine. Urinary ketamine and norketamine levels were relatively low at 4-24 h intervals and were difficult to assay in a normal laboratory. In the present study, the determination of urinary dehydronorketamine levels at 2-24 hours appears to have a great potential for use in Schedule III controlled drugs management.

AB - A liquid chromatography/atmospheric pressure ionization mass spectrometry has been developed for the determination of ketamine, norketamine, and dehydronorketamine in human urine. A separation of these analytes in urine samples without tedious pretreatment procedures has been achieved within 10 min. Linear calibration curves of these analytes with coefficients better than 0.998 have been obtained over a wide range from 12.5 to 200 ng/mL. The accuracy was between 2.1% and 7.2% with detection limits at levels of 0.02 ng/mL, 0.02 ng/mL and 0.93 ng/mL for ketamine, norketamine and dehydronorketamine, respectively. The results demonstrate the suitability of the liquid chromatography/atmospheric pressure ionization mass spectrometry approach to analyze trace ketamine, norketamine and dehydronorketamine in urine. Urinary ketamine and norketamine levels were relatively low at 4-24 h intervals and were difficult to assay in a normal laboratory. In the present study, the determination of urinary dehydronorketamine levels at 2-24 hours appears to have a great potential for use in Schedule III controlled drugs management.

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