Simultaneous dynamic stiffness and extension profiles of single titin molecules: Nanomechanical evidence for unfolding intermediates

Jeffrey G. Forbes, Kuan Wang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The stiffness and extension force of single native titin molecules were measured. A molecular imaging PicoSPM AFM with a MAC-mode attachment and breakout box was augmented with an EG&G 7260 lockin amplifier and a Stanford Research Systems SR650 filter to measure force-extension and stiffness-extension curves simultaneously. It was shown that the acquisition of both force and stiffness on single protein molecules molecules was expected to provide new insights into folding intermediate states and elastic force generation in muscle and other soft biological composite material. The amplitude signal from the lockin was buffered and digitized by the auxiliary input on the PicoSPM control electronics.

Original languageEnglish
Pages (from-to)1439-1443
Number of pages5
JournalJournal of Vacuum Science and Technology A: Vacuum, Surfaces and Films
Volume22
Issue number4
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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