Simultaneous Determination of Podophyllotoxin, Quercetin and Kaempferol in Podophyllin by Liquid Chromatography Tandem Mass Spectrometry

Mei-Chih Lin, Jer-Huei Lin, Shu-Kong Chen, Yu-Wen Cheng, Huei-Wen Cheng

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Abstract

A quantitative high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) was developed to analyze the constituents of podophyllin. Chromatographic separation was performed on a Cosmosil 5C18-MS (25 cm × 4.6 mm ID., 5 μm) reverse phase column using a gradient of mobile phase (0.25% formic acid-methanol). The column effluent was split 2: 3 into the photodiode detector and tandem mass spectrometer. Podophyllotoxin, quercetin and kaempferol in podophyllin were identified by daughter ion scan mode and then determined their contents by multiple-reaction monitoring (MRM) mode. The limits of detection and quantitation for podophyllotoxin, quercetin and kaempferol were 2.40, 8.01; 2.94, 9.87 and 3.10, 10.2 ng/mL, respectively. The relative standard deviations of intraday and interday analyses for podophyllotoxin, quercetin and kaempferol ranged from 0.52-6.01% and 2.48-9.88%, respectively. The mean recoveries for podophyllotoxin, quercetin and kaempferol were 101.4%, 98.3% and 98.7%, respectively. The developed LC/MS/MS method was suitable for the simultaneous determinations of podophyllotoxin, quercetin and kaempferol in podophyllin.
Original languageUndefined/Unknown
Pages (from-to)29-40
Number of pages12
JournalJournal of Food and Drug Analysis
Volume16
Issue number6
Publication statusPublished - 2008

Keywords

  • 液相層析串聯式質譜法
  • 鬼臼樹脂Podophyllin
  • podophyllotoxin
  • quercetin
  • kaempferol
  • LC/MS/MS
  • podophyllin

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