Simultaneous determination of ampicillin, cefoperazone, and sulbactam in pharmaceutical formulations by HPLC with β-cyclodextrin stationary phase

Tai Li Tsou, Yu Chuan Huang, Chiu Wey Lee, An Rong Lee, Hsian-Jenn Wang, Su Hwei Chen

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27 Citations (Scopus)


An accurate and reproducible method for the simultaneous determination of ampicillin (AMP), sulbactam (SUL), and cefoperazone (CFP) in pharmaceutical formulations by using HPLC with β-CD stationary phase was developed. It involved the use of the added tetraethylammonium acetate (TEAA) reagent, pH, and methanol as the significant parameters to find the optimum separation condition. A high resolution and selectivity of analytes was obtained by running the mobile phase in methanol-5 mM TEAA buffer = 35:65 (v/v, pH 4.5) at 280 nm. The mean recoveries ranged from 96.6 to 103.3% for AMP in the synthetic mixture, 97.6 to 103.0% for SUL, and 97.0 to 104.0% for CFP. The low LOD (<1.8 μg/mL) and low CV (<0.9%) assured that this method was sensitive and reproducible. The assay of analytes in commercial products exhibited that it was convenient and reproducible for routine analyses of these components in sterilized H2O, saline, or 5% dextrose injection solutions.

Original languageEnglish
Pages (from-to)2407-2413
Number of pages7
JournalJournal of Separation Science
Issue number15
Publication statusPublished - Oct 1 2007
Externally publishedYes



  • β-Cyclodextrin
  • Ampicillin
  • Cefoperazone
  • HPLC assay
  • Sulbactam

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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