Simultaneous detection and quantitation of highly water-soluble herbicides in serum using ion-pair liquid chromatography-tandem mass spectrometry

Kuang Chuan Wang, Shih Ming Chen, Jung Fa Hsu, Sheaw Guey Cheng, Ching Kuo Lee

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We report the simultaneous screening of highly polar, water-soluble, and less-volatile herbicides, including glyphosate, glufosinate, paraquat, and diquat, in serum using liquid chromatography-mass spectrometry. The herbicides were separated by solid-phase extraction using a Strata-XC cartridge. A heptafluorobutyric acid solution was chosen as the mobile phase for ion-pair liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The serum specimens were screened for the presence of the herbicides at the following concentrations: 5 ng/mL for glyphosate, 2 ng/mL for glufosinate, 1 ng/mL for diquat, and 5 ng/mL for paraquat. This is the first report on the simultaneous detection of these compounds.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume876
Issue number2
DOIs
Publication statusPublished - Dec 15 2008

Fingerprint

glyphosate
Ion chromatography
Liquid chromatography
Herbicides
Diquat
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Paraquat
Ions
Water
Mass Spectrometry
Serum
Solid Phase Extraction
Screening
phosphinothricin

Keywords

  • Glyphosate
  • Herbicides
  • HFBA
  • Ion-pair
  • LC-MS
  • Paraquat

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

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abstract = "We report the simultaneous screening of highly polar, water-soluble, and less-volatile herbicides, including glyphosate, glufosinate, paraquat, and diquat, in serum using liquid chromatography-mass spectrometry. The herbicides were separated by solid-phase extraction using a Strata-XC cartridge. A heptafluorobutyric acid solution was chosen as the mobile phase for ion-pair liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The serum specimens were screened for the presence of the herbicides at the following concentrations: 5 ng/mL for glyphosate, 2 ng/mL for glufosinate, 1 ng/mL for diquat, and 5 ng/mL for paraquat. This is the first report on the simultaneous detection of these compounds.",
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AU - Wang, Kuang Chuan

AU - Chen, Shih Ming

AU - Hsu, Jung Fa

AU - Cheng, Sheaw Guey

AU - Lee, Ching Kuo

PY - 2008/12/15

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KW - LC-MS

KW - Paraquat

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