TY - JOUR
T1 - Simultaneous detection and quantitation of highly water-soluble herbicides in serum using ion-pair liquid chromatography-tandem mass spectrometry
AU - Wang, Kuang Chuan
AU - Chen, Shih Ming
AU - Hsu, Jung Fa
AU - Cheng, Sheaw Guey
AU - Lee, Ching Kuo
PY - 2008/12/15
Y1 - 2008/12/15
N2 - We report the simultaneous screening of highly polar, water-soluble, and less-volatile herbicides, including glyphosate, glufosinate, paraquat, and diquat, in serum using liquid chromatography-mass spectrometry. The herbicides were separated by solid-phase extraction using a Strata-XC cartridge. A heptafluorobutyric acid solution was chosen as the mobile phase for ion-pair liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The serum specimens were screened for the presence of the herbicides at the following concentrations: 5 ng/mL for glyphosate, 2 ng/mL for glufosinate, 1 ng/mL for diquat, and 5 ng/mL for paraquat. This is the first report on the simultaneous detection of these compounds.
AB - We report the simultaneous screening of highly polar, water-soluble, and less-volatile herbicides, including glyphosate, glufosinate, paraquat, and diquat, in serum using liquid chromatography-mass spectrometry. The herbicides were separated by solid-phase extraction using a Strata-XC cartridge. A heptafluorobutyric acid solution was chosen as the mobile phase for ion-pair liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The serum specimens were screened for the presence of the herbicides at the following concentrations: 5 ng/mL for glyphosate, 2 ng/mL for glufosinate, 1 ng/mL for diquat, and 5 ng/mL for paraquat. This is the first report on the simultaneous detection of these compounds.
KW - Glyphosate
KW - HFBA
KW - Herbicides
KW - Ion-pair
KW - LC-MS
KW - Paraquat
UR - http://www.scopus.com/inward/record.url?scp=56749102635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56749102635&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2008.10.042
DO - 10.1016/j.jchromb.2008.10.042
M3 - Article
C2 - 19022710
AN - SCOPUS:56749102635
SN - 1570-0232
VL - 876
SP - 211
EP - 218
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -