Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study

Yi Chuan Chen, Li Chern Pan, Cheng Wei Lai, Ying Shan Chien, Tzu Hua Wu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glucose is suggested to play a key role in motility hyperactivation of mammalian spermatozoa. The current study aimed to investigate the modulatory effects of silymarin and/or a protein kinase A (PKA) inhibitor (H-89) on glucose-mediated motility parameters of mouse spermatozoa. Spermatozoa were incubated in HEPES medium containing normal (NG; 5.5 mM) or high (HG; 25 mM) glucose concentration. The results of computer-assisted analysis showed that samples incubated in HG resulted in a larger (p<0.05) percentage of motile spermatozoa at 120 min (59.5 ± 14.8% vs. 34.0 ± 4.4%) compared to those incubated in NG. The average pathway velocity (VAP), curvilinear velocity (VCL), and straight-line velocity (VSL) exhibited similar patterns at 60 and 120 min when incubated in HG (p<0.05). Treatments with silymarin (5, 10, 20 μg/mL) did not significantly affect sperm motility under NG conditions, but decreased the HG-enhanced motility, VAP, and VCL at 120. min (p<0.05). H-89 (30 μM) reduced (p<0.05) motility at 30 min examined in the NG or HG medium. At 90 min, H-89 also reduced (p<0.05) the HG-enhanced motility of spermatozoa incubated with or without 20 μg/mL silymarin by 49% or 32%, respectively. In conclusion, the H-89-inhibition of glucose-mediated mouse sperm motility and certain types of velocity suggests that the glycolysis-PKA pathway is involved in the regulation of sperm motility. Silymarin may maintain sperm motility under NG conditions, but it inhibits glucose-activated sperm motility.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalReproductive biology
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Silymarin
silymarin
cAMP-dependent protein kinase
Sperm Motility
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
sperm motility
in vitro studies
Spermatozoa
Glucose
glucose
mice
spermatozoa
HEPES
Glycolysis
glycolysis
In Vitro Techniques
N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide

Keywords

  • Computer-assisted semen analysis
  • Glucose
  • Mouse
  • Protein kinase A pathway
  • Sperm motility parameters

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology
  • Endocrinology

Cite this

Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility : An in vitro study. / Chen, Yi Chuan; Pan, Li Chern; Lai, Cheng Wei; Chien, Ying Shan; Wu, Tzu Hua.

In: Reproductive biology, Vol. 15, No. 3, 01.09.2015, p. 172-177.

Research output: Contribution to journalArticle

@article{7a5ced677f7649db8a651e0ef3b741e9,
title = "Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study",
abstract = "Glucose is suggested to play a key role in motility hyperactivation of mammalian spermatozoa. The current study aimed to investigate the modulatory effects of silymarin and/or a protein kinase A (PKA) inhibitor (H-89) on glucose-mediated motility parameters of mouse spermatozoa. Spermatozoa were incubated in HEPES medium containing normal (NG; 5.5 mM) or high (HG; 25 mM) glucose concentration. The results of computer-assisted analysis showed that samples incubated in HG resulted in a larger (p<0.05) percentage of motile spermatozoa at 120 min (59.5 ± 14.8{\%} vs. 34.0 ± 4.4{\%}) compared to those incubated in NG. The average pathway velocity (VAP), curvilinear velocity (VCL), and straight-line velocity (VSL) exhibited similar patterns at 60 and 120 min when incubated in HG (p<0.05). Treatments with silymarin (5, 10, 20 μg/mL) did not significantly affect sperm motility under NG conditions, but decreased the HG-enhanced motility, VAP, and VCL at 120. min (p<0.05). H-89 (30 μM) reduced (p<0.05) motility at 30 min examined in the NG or HG medium. At 90 min, H-89 also reduced (p<0.05) the HG-enhanced motility of spermatozoa incubated with or without 20 μg/mL silymarin by 49{\%} or 32{\%}, respectively. In conclusion, the H-89-inhibition of glucose-mediated mouse sperm motility and certain types of velocity suggests that the glycolysis-PKA pathway is involved in the regulation of sperm motility. Silymarin may maintain sperm motility under NG conditions, but it inhibits glucose-activated sperm motility.",
keywords = "Computer-assisted semen analysis, Glucose, Mouse, Protein kinase A pathway, Sperm motility parameters",
author = "Chen, {Yi Chuan} and Pan, {Li Chern} and Lai, {Cheng Wei} and Chien, {Ying Shan} and Wu, {Tzu Hua}",
year = "2015",
month = "9",
day = "1",
doi = "10.1016/j.repbio.2015.06.003",
language = "English",
volume = "15",
pages = "172--177",
journal = "Reproductive biology",
issn = "1642-431X",
publisher = "Elsevier Urban and Partner sp. z o.o.",
number = "3",

}

TY - JOUR

T1 - Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility

T2 - An in vitro study

AU - Chen, Yi Chuan

AU - Pan, Li Chern

AU - Lai, Cheng Wei

AU - Chien, Ying Shan

AU - Wu, Tzu Hua

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Glucose is suggested to play a key role in motility hyperactivation of mammalian spermatozoa. The current study aimed to investigate the modulatory effects of silymarin and/or a protein kinase A (PKA) inhibitor (H-89) on glucose-mediated motility parameters of mouse spermatozoa. Spermatozoa were incubated in HEPES medium containing normal (NG; 5.5 mM) or high (HG; 25 mM) glucose concentration. The results of computer-assisted analysis showed that samples incubated in HG resulted in a larger (p<0.05) percentage of motile spermatozoa at 120 min (59.5 ± 14.8% vs. 34.0 ± 4.4%) compared to those incubated in NG. The average pathway velocity (VAP), curvilinear velocity (VCL), and straight-line velocity (VSL) exhibited similar patterns at 60 and 120 min when incubated in HG (p<0.05). Treatments with silymarin (5, 10, 20 μg/mL) did not significantly affect sperm motility under NG conditions, but decreased the HG-enhanced motility, VAP, and VCL at 120. min (p<0.05). H-89 (30 μM) reduced (p<0.05) motility at 30 min examined in the NG or HG medium. At 90 min, H-89 also reduced (p<0.05) the HG-enhanced motility of spermatozoa incubated with or without 20 μg/mL silymarin by 49% or 32%, respectively. In conclusion, the H-89-inhibition of glucose-mediated mouse sperm motility and certain types of velocity suggests that the glycolysis-PKA pathway is involved in the regulation of sperm motility. Silymarin may maintain sperm motility under NG conditions, but it inhibits glucose-activated sperm motility.

AB - Glucose is suggested to play a key role in motility hyperactivation of mammalian spermatozoa. The current study aimed to investigate the modulatory effects of silymarin and/or a protein kinase A (PKA) inhibitor (H-89) on glucose-mediated motility parameters of mouse spermatozoa. Spermatozoa were incubated in HEPES medium containing normal (NG; 5.5 mM) or high (HG; 25 mM) glucose concentration. The results of computer-assisted analysis showed that samples incubated in HG resulted in a larger (p<0.05) percentage of motile spermatozoa at 120 min (59.5 ± 14.8% vs. 34.0 ± 4.4%) compared to those incubated in NG. The average pathway velocity (VAP), curvilinear velocity (VCL), and straight-line velocity (VSL) exhibited similar patterns at 60 and 120 min when incubated in HG (p<0.05). Treatments with silymarin (5, 10, 20 μg/mL) did not significantly affect sperm motility under NG conditions, but decreased the HG-enhanced motility, VAP, and VCL at 120. min (p<0.05). H-89 (30 μM) reduced (p<0.05) motility at 30 min examined in the NG or HG medium. At 90 min, H-89 also reduced (p<0.05) the HG-enhanced motility of spermatozoa incubated with or without 20 μg/mL silymarin by 49% or 32%, respectively. In conclusion, the H-89-inhibition of glucose-mediated mouse sperm motility and certain types of velocity suggests that the glycolysis-PKA pathway is involved in the regulation of sperm motility. Silymarin may maintain sperm motility under NG conditions, but it inhibits glucose-activated sperm motility.

KW - Computer-assisted semen analysis

KW - Glucose

KW - Mouse

KW - Protein kinase A pathway

KW - Sperm motility parameters

UR - http://www.scopus.com/inward/record.url?scp=84941314349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941314349&partnerID=8YFLogxK

U2 - 10.1016/j.repbio.2015.06.003

DO - 10.1016/j.repbio.2015.06.003

M3 - Article

C2 - 26370460

AN - SCOPUS:84941314349

VL - 15

SP - 172

EP - 177

JO - Reproductive biology

JF - Reproductive biology

SN - 1642-431X

IS - 3

ER -