Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells

Chin Lin Huang, I. Lun Hsiao, Ho Chen Lin, Chu Fang Wang, Yuh Jeen Huang, Chun Yu Chuang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Silver nanoparticles (AgNPs) have antibacterial characteristics, and currently are applied in Ag-containing products. This study found neural cells can uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene expression of inflammation and neurodegenerative disorder in murine brain ALT astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5. μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor with collagenous structure (MARCO) and glutathione synthetase (GSS) for inflammatory response and oxidative stress neutralization. Additionally, this study found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD) deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene expression of amyloid precursor protein (APP) was induced, and otherwise, neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural cells as well as protein level. These results suggested AgNPs could alter gene and protein expressions of Aβ deposition potentially to induce AD progress in neural cells. It's necessary to take notice of AgNPs distribution in the environment.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalEnvironmental Research
Volume136
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

Fingerprint

Silver
Gene expression
Nanoparticles
gene expression
brain
silver
Brain
Gene Expression
protein
Glutathione Synthase
Neprilysin
Oxidative stress
Amyloid beta-Protein Precursor
LDL Receptors
Macrophages
neutralization
Interleukin-1
Chemokines
Amyloid
secretion

Keywords

  • Alzheimer 's disease
  • Gene expression
  • Inflammation
  • Neurodegenerative disorder
  • Silver nanoparticle

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

Cite this

Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells. / Huang, Chin Lin; Hsiao, I. Lun; Lin, Ho Chen; Wang, Chu Fang; Huang, Yuh Jeen; Chuang, Chun Yu.

In: Environmental Research, Vol. 136, 01.01.2015, p. 253-263.

Research output: Contribution to journalArticle

Huang, Chin Lin ; Hsiao, I. Lun ; Lin, Ho Chen ; Wang, Chu Fang ; Huang, Yuh Jeen ; Chuang, Chun Yu. / Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells. In: Environmental Research. 2015 ; Vol. 136. pp. 253-263.
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