Abstract

Autophagy is a survival mechanism that is activated in response to nutrient deprivation. The link between aberrant autophagy and cancer has been increasingly recognized. Survivin, an anti-apoptotic molecule, and the autophagy pathway are correlated with therapeutic responses to cancer. However, the role of autophagy in cancer progression remains unclear. Here, we generated survivin knockdown cells (survivin-KD) by introducing a short interfering RNA (siRNA) into hepatocellular carcinoma (HCC) cells, and we observed a 20 % reduction in the survival of these survivin- KD cells, as determined by MTT assay. In addition, an increased number of stress granules, increased positive staining by acridine orange and a shift in the high side scatter (SSC) cell population in flow cytometry analysis were observed in survivin-KD cells. Furthermore, electronmicroscopy revealed an increased number of autophagosomes in survivin-KD cells compared with scrambled control cells. Finally, we treated cells with an autophagy inhibitor, 3-MA, and observed a decrease in cell survival in survivin-KD cells compared with scrambled control cells. Our study suggests that an autophagy signal may be activated after the anti-apoptotic molecule survivin is suppressed. This finding implies that autophagy may be an alternative survival pathway in HCC cells and may provide a basis for the development of new therapeutic strategies for HCC.

Original languageEnglish
Pages (from-to)9957-9966
Number of pages10
JournalTumor Biology
Volume35
Issue number10
DOIs
Publication statusPublished - Jul 10 2014

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Autophagy
Hepatocellular Carcinoma
Side-Population Cells
Neoplasms
Acridine Orange
Small Interfering RNA
Cell Survival
Flow Cytometry

Keywords

  • Anti-proliferation
  • Autophagy
  • HCC
  • Survivin

ASJC Scopus subject areas

  • Cancer Research
  • Medicine(all)

Cite this

Silencing survivin activates autophagy as an alternative survival pathway in HCC cells. / Chang, Yu Jia; Li, Li Tzu; Chen, Hsin An; Hung, Chin Sheng; Wei, Po Li.

In: Tumor Biology, Vol. 35, No. 10, 10.07.2014, p. 9957-9966.

Research output: Contribution to journalArticle

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