Abstract

Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.

Original languageEnglish
Article numbere006157
JournalJournal of the American Heart Association
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Mitochondrial DNA
Leukocytes
Stroke
Deoxyguanosine
Glutathione Disulfide
Oxidative Stress
Logistic Models
Glutathione
Real-Time Polymerase Chain Reaction
Atherosclerosis
Diabetes Mellitus
Smoking
Enzyme-Linked Immunosorbent Assay

Keywords

  • Association study
  • Ischemic stroke
  • Mitochondrial DNA content

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke. / Lien, Li Ming; Chiou, Hung Yi; Yeh, Hsu Ling; Chiu, Shang Yen; Jeng, Jiann Shing; Lin, Huey Juan; Hu, Chaur Jong; Hsieh, Fang I.; Wei, Yau Huei.

In: Journal of the American Heart Association, Vol. 6, No. 11, e006157, 01.11.2017.

Research output: Contribution to journalArticle

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abstract = "Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.",
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T1 - Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke

AU - Lien, Li Ming

AU - Chiou, Hung Yi

AU - Yeh, Hsu Ling

AU - Chiu, Shang Yen

AU - Jeng, Jiann Shing

AU - Lin, Huey Juan

AU - Hu, Chaur Jong

AU - Hsieh, Fang I.

AU - Wei, Yau Huei

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.

AB - Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.

KW - Association study

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